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Submitted: 12 Jul 2023
Revision: 14 Jan 2024
Accepted: 31 Jan 2024
ePublished: 30 Mar 2024
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Avicenna J Clin Microbiol Infect. 2024;11(1): 22-27.
doi: 10.34172/ajcmi.3474
  Abstract View: 429
  PDF Download: 167

Original Article

Evaluation of Antimicrobial Susceptibility in Extended-Spectrum β-Lactamase Producing Escherichia coli Isolates From Patients in Northwest Iran

Mohammad Bahloli 1 ORCID logo, Hossein Samadi Kafil 2, Parisa Roshani Asl 3, Niloufar Rashidi 3* ORCID logo, Seyyed Reza Moaddab 4* ORCID logo

1 Department of Hematology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
2 Department of Microbiology, Faculty Medicine, Tabriz University of Medical Science, Tabriz, Iran
3 Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
4 Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Tabriz University of Medical Science, Tabriz, Iran
*Corresponding Authors: Niloufar Rashidi, Email: ni.rashidi@gmail.com; Seyyed Reza Moaddab, Email: srmoaddab@gmail.com

Abstract

Background: Escherichia coli is one of the main causes of various diseases worldwide, whose multidrug-resistant strains have caused many public health problems by producing extended-spectrum β-lactamases (ESBLs). The resistance rate varies in different regions. Thus, it is necessary to identify ESBL-producing strains in each region and their antibiotic sensitivity in order to find appropriate treatment options. Hence, the present study aimed to detect the ESBL-producing E. coli strains and their antimicrobial susceptibility pattern in Tabriz, Iran.

Methods: This study was conducted at the Imam Reza Hospital in Tabriz from November 20, 2022, to April 20, 2023. A total of 400 E. coli isolates were collected from different clinical specimens. Antimicrobial susceptibility testing was performed by the disk diffusion method. ESBL-producing isolates were detected by the double-disc synergy test method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines.

Results: Out of 400 E. coli isolates, 211 (52.75%) were obtained from females, and 189 (47.25%) belonged to males. The mean age of patients was 52.1±27.9 years. Overall, 279 (69.75%) were confirmed as ESBL producers. These producers were mainly recovered from outpatients. The highest antibiotic resistance was observed to ceftriaxone (86.25%) and tetracycline (80.75%), and the least antibiotic resistance was related to imipenem (8%) and amikacin (16.25%), respectively. The rate of antibiotic resistance among ESBL producers was higher than among non-ESBLs.

Conclusion: The present study reported a high prevalence of ESBL-producing E. coli among patients referring to Imam Reza hospital in Tabriz. Carbapenems, aminoglycosides, and nitrofurantoins were confirmed as the most efficient drugs for these bacteria, whereas cephalosporins, fluoroquinolones, and sulfonamides were the least effective agents.


Please cite this article as follows: Bahloli M, Samadi Kafil H, Roshani Asl P, Rashidi N, Moaddab SR. Evaluation of Antimicrobial Susceptibility in Extended-Spectrum β-Lactamase Producing Escherichia coli Isolates From Patients in Northwest Iran. Avicenna J Clin Microbiol Infect. 2024; 11(1):22-27. doi:10.34172/ajcmi.3474
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