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Submitted: 31 Oct 2020
Accepted: 16 Nov 2019
ePublished: 31 Dec 2019
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Avicenna J Clin Microbiol Infect. 2019;6(4): 122-126.
doi: 10.34172/ajcmi.2019.22
  Abstract View: 1785
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Original Article

Detection of Brucella Antibodies in Dogs From Rural Regions of Hamedan, Iran

Jamal Gharekhani 1,2* ORCID logo, Alireza Sazmand 3 ORCID logo

1 Department of Laboratory Sciences, Central Veterinary Laboratory, Iranian Veterinary Organization, Hamedan, Iran.
2 Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
3 Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran.
*Corresponding Author: *Corresponding author: Jamal Gharekhani, Central Veterinary Laboratory, Hamedan Veterinary Office, Ayatollah-Rafsanjani Street, Hamedan, Iran , Postal Code: 6519611156 Tel: + (98)81 32651801, Fax: + (98)81 32644474, E-mail: , Email: gharekhani_76@yahoo.com

Abstract

Background: Dogs play a significant role in the maintenance of various pathogens in the environment and their possible transmission to humans. In the case of Brucella spp., infected dogs can shed organisms into the environment via urine and vaginal discharges, and aborted materials or feces. This study aimed to investigate the seroprevalence of Brucella sp. infection in dogs in the rural regions of Hamedan, western Iran.

Methods: Between June and November 2018, Blood samples were obtained from cephalic or saphenous veins of 180 stray dogs from 6 rural regions of Hamedan during June and November 2018. The sera samples were evaluated for the presence of antibodies against Brucella spp. using Rose Bengal plate test (RBT) and Wright’s serum agglutination test (Wright SAT).

Results: Seroprevalence rate of Brucella infection was 3.3% by RBT. (6/180; 95% CI: 0.7%–5.9%). All of the serum positive dogs had titers of 1:80 by Wright SAT. The seropositivity was 3.1% in males, 3.4% in females, 3.2% in <1-year-old, 1.8% in 1–2-year-old, and 4.9% in >2-year-old dogs. No statistically significant correlation was found between the infection rate and gender of dogs (P=0.907) or age groups (P=0.772).

Conclusions: The presence of infected dogs in rural regions is an important risk factor for the transmission of Brucella to humans and livestock. It is suggested that villagers, shepherds, and their families especially children should be provided with the information about risks of getting infection when handling an infected dog.

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