Logo-ajcmi
Submitted: 19 Apr 2024
Revision: 30 May 2024
Accepted: 07 Jun 2024
ePublished: 30 Jun 2024
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)

Avicenna J Clin Microbiol Infect. 2024;11(2): 74-81.
doi: 10.34172/ajcmi.3543
  Abstract View: 169
  PDF Download: 66

Original Article

Antibacterial Properties of Mint Nanoemulsion Against Uropathogenic Escherichia coli Carrying Beta-Lactamase Resistance Genes

Hala Maitham Saker Alali 1 ORCID logo, Ashraf Kariminik 2,3* ORCID logo, Maryam Ghane 4

1 Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Microbiology, Kerman Branch, Islamic Azad University, Kerman, Iran
3 Food and Agricultural Safety Research Center, Kerman Branch, Islamic Azad University, Kerman, Iran
4 Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran
*Corresponding Author: Ashraf Kariminik, Email: a.kariminik@iauk.ac.ir

Abstract

Background: Uropathogenic Escherichia coli (UPEC) is a significant contributor to urinary tract infections (UTIs). The inappropriate prescription and utilization of antibiotics in treating UTIs have resulted in a rise in antibiotic resistance. The aim of this study was to investigate the antibacterial properties of mint nanoemulsion against beta-lactam-resistant strains of UPEC.

Methods: Three hundred fifty urine samples were collected in sterile containers and cultured on eosin-methylene blue agar and blood agar media under sterile conditions. Microbial sensitivity testing was conducted using the standard disk diffusion method. Molecular analysis was performed to identify the blaTEM, blaSHV, and blaCTX genes in the samples. The mint nanoemulsion was produced using the low-energy method of spontaneous emulsion formation and characterized using scanning electron microscopy (SEM), dynamic light scattering (DLS), and Fourier-transform infrared spectroscopy (FTIR). The antibacterial activity of the mint nanoemulsion against multidrug-resistant (MDR) isolates underwent assessment.

Results: One hundred UPEC strains were isolated and identified, showing differing levels of resistance and sensitivity to various antibiotics. Nitrofurantoin exhibited the highest resistance (22%), while amikacin had the lowest resistance (4%). The prevalence of the blaTEM, blaSHV, and blaCTX genes among the UPEC isolates was 93.33%, 100%, and 73.33%, respectively. The mint nanoemulsion demonstrated effective antibacterial activity against resistant isolates, with a minimum inhibitory concentration of 0.2 mg/µL and a minimum bactericidal concentration of 0.3 mg/µL.

Conclusion: The resistance of UPEC against conventional antibiotics commonly used to treat UTIs was consistently high and demonstrated an increasing trend over time. This escalating situation could potentially become more severe in the future if the rational consumption of antibiotics is not carefully managed. Nanoemulsions synthesized using mint revealed promising stability and robust antimicrobial properties against UPEC isolates. Consequently, it is evident that these synthesized nanoemulsions represent a viable candidate as effective antimicrobial agents in combating antibiotic resistance in UTI treatment.


Please cite this article as follows: Maitham Saker Alali H, Kariminik A, Ghane M. Antibacterial properties of mint nanoemulsion against uropathogenic Escherichia coli carrying beta-lactamase resistance genes. Avicenna J Clin Microbiol Infect. 2024; 11(2):74-81. doi:10.34172/ ajcmi.3543
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 170

Your browser does not support the canvas element.


PDF Download: 66

Your browser does not support the canvas element.