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Submitted: 17 Oct 2023
Revision: 14 Jan 2024
Accepted: 14 Feb 2024
ePublished: 16 Jun 2024
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Avicenna J Clin Microbiol Infect. Inpress.
doi: 10.34172/ajcmi.3504
  Abstract View: 16

Original Article

Molecular detection of cylindrospermopsin producing cyanobacteria in the coasts of Hormozgan province

Elmira Eivazpour Nami ORCID logo, Mohammad Hassan Shah Hoseini* ORCID logo, Alireza Dehnad
*Corresponding Author: Email: shahhosseiny@yahoo.com

Abstract

Background: In recent years, water and environmental pollution by cyanobacteria are being increasingly commonly reported in the world that is a serious hazard for human health. Cylindrospermopsin is one of the most important algal toxin produced by cyanobacteria that cause damage to the liver, kidneys, thymus and heart in human. The aim of this study was to accurate and fast detection of cylindrospermopsin toxigenic cyanobacteria in the water resources of the Hormozgan province coast by molecular method. Methods: We collected 20 water samples (2 liters) from twenty sampling stations of the coasts of Hormozgan province in October 2017. The extraction of genomic DNA was performed from water samples. Finally, molecular detection was conducted by polymerase chain reaction (PCR) method. Results: The optimized PCR successfully detected 20 cyanobacteria strain from all twenty samples. In addition, presence of cylindrospermopsin toxigenic cyanobacteria was confirmed at 2 (10%) stations Conclusions: Generally, our study confirmed the risk of toxigenic cyanobacteria presence in the coasts of Hormozgan province, and demonstrated that PCR assay is an accurate and fast method for early detection of cylindrospermopsin toxigenic cyanobacteria from water resources.
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