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Submitted: 05 Sep 2023
Revision: 15 Sep 2023
Accepted: 20 Sep 2023
ePublished: 29 Sep 2023
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Avicenna J Clin Microbiol Infect. 2023;10(3): 120-125.
doi: 10.34172/ajcmi.3496
  Abstract View: 520
  PDF Download: 287

Original Article

Antimicrobial Photodynamic Therapy Using Zinc Phthalocyanine Nanoemulsion Against Infected Wounds in Diabetic Rats

Sara Soleymani Sedeh 1 ORCID logo, Mahnoosh Fatemi 2* ORCID logo, Fereshte Ghandehari 1 ORCID logo

1 Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2 Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
*Corresponding Author: Mahnoosh Fatemi, Email: Mehr.fatemi7@gmail.com

Abstract

Background: Chronic diabetic infections commonly involve highly antibiotic-resistant pathogens, such as Staphylococcus aureus and streptococci. This study aimed to assess the impact of zinc (Zn) phthalocyanine incorporated into nanoemulsions on diabetic wound infections.

Methods: Thirty-six adult male rats were divided into control, diabetic wound, diabetic wound infected with S. aureus, and three diabetic wound infected with S. aureus groups, which were treated with laser, medication, and a combination of medication and laser therapy. After the treatment period, wound diameter was measured, and blood samples and wound tissue were collected to evaluate antioxidant factors.

Results: The results demonstrated a significant reduction in wound diameter in the group treated with both the drug and laser compared to the other groups. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX), along with the concentration of glutathione (GSH) in the blood of all groups, exhibited a significant decrease in comparison to the control group. However, the activity of these factors in both blood and tissue showed a noteworthy increase in the rats treated with both the drug and laser, as opposed to diabetic rats infected with S. aureus.

Conclusion: Photodynamic therapy (PDT) for infectious wounds, employing nanoemulsions containing Zn phthalocyanine, appears to enhance the body’s antioxidant system by eradicating bacteria and, ultimately, expediting wound healing. This approach may be considered a potential candidate for treating antibiotic-resistant infections.


Please cite this article as follows: Soleymani Sedeh S, Fatemi M, Ghandehari F. Antimicrobial photodynamic therapy using zinc phthalocyanine nanoemulsion against infected wounds in diabetic rats. Avicenna J Clin Microbiol Infect. 2023; 10(3):120-125. doi:10.34172/ajcmi.3496
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