Logo-ajcmi
Submitted: 19 Mar 2023
Accepted: 23 Mar 2023
ePublished: 29 Mar 2023
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)

Avicenna J Clin Microbiol Infect. 2023;10(1): 9-12.
doi: 10.34172/ajcmi.2023.3449
  Abstract View: 255
  PDF Download: 165

Original Article

Investigation and Follow-up of Brucellosis in Seropositive Patients and Their Families in Hamadan Province, Iran

Mohammad Yousef Alikhani 1,2* ORCID logo, Maryam Adabi 1, Fariba Keramat 1,3 ORCID logo, Abbas Moghimbaigi 1 ORCID logo, Sima Kazemi 2 ORCID logo, Somaye Shiralizadeh 2 ORCID logo

1 Infectious Disease Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
2 Microbiology Department, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3 Department of Infectious Diseases, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
*Corresponding Author: Mohammad Yousef Alikhani, Emails: alikhani43@yahoo. com, , Email: alikhani@umsha.ac.ir

Abstract

Background: Brucellosis is recognized as one of the most prevalent diseases among humans and animals. This study investigated and followed up brucellosis in seropositive participants in the Famenin (Hamadan province, Iran) cohort of brucellosis and their families by culture and serology methods.

Methods: Blood samples were taken from 66 subjects, including 18 subjects in the Famenin brucellosis cohort study with antibody titers≥1:180 and 36 subjects from their families and 12 subjects in the Famenin brucellosis cohort study with antibody titers<1:80. In the serological method, standard tube agglutination test (STAT positive with≥1:80) and 2-mercaptoethanol (2-ME) test (positive with≥1:40) were performed using the patient serum. Finally, 8 cc of the blood of all subjects was used for culture in the BACTEC culture medium.

Results: Of the 66 serum samples, 20 (30.3%) samples, including 5, 4, and 10 samples at 1:20, 1:40, and 1:80 dilution, respectively, and 1 sample at 1:160 dilution were positive by the STAT, of which 13 (65%) samples belonged to patients’ family members. Using the 2-ME test, 10 (15.2%) serum samples were positive, of which 5 (50%) cases were related to patients’ family members. Eventually, no growth of Brucella was observed in 66 flasks of the BACTEC culture medium.

Conclusion: Considering that a definite diagnostic method is not yet accessible, a combination of methods must be applied to diagnose the disease.


Please cite this article as follows: Alikhani MY, Adabi M, Keramat F, Moghimbaigi A, Kazemi S, Shiralizadeh S. Investigation and follow-up of brucellosis in seropositive patients and their families in Hamadan province, Iran. Avicenna J Clin Microbiol Infect. 2023; 10(1):9-12. doi:10.34172/ajcmi.2023.3449
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 256

Your browser does not support the canvas element.


PDF Download: 165

Your browser does not support the canvas element.