Submitted: 18 Jun 2022
Revision: 31 Jul 2022
Accepted: 08 Aug 2022
ePublished: 29 Sep 2022
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Avicenna J Clin Microbiol Infect. 2022;9(3): 119-123.
doi: 10.34172/ajcmi.2022.3393
  Abstract View: 385
  PDF Download: 283

Original Article

Evaluation of Anti-leishmanial Effect of Selenium Nanoparticles on Leishmania major Promastigotes In Vitro

Roghayeh Norouzi 1 ORCID logo, Farzaneh Mirzaei 2 ORCID logo, Abolghasem Siyadatpanah 3 ORCID logo, Seyed Jafar Adnani Sadati 4,5* ORCID logo

1 Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
2 Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3 Ferdows School of Paramedical and Health, Birjand University of Medical Sciences, Birjand, Iran
4 Department of Microbiology Immunology and Parasitology, School of Medicine, Qom University of Medical Sciences, Qom, Iran
5 Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran
*Corresponding Author: Corresponding author: Seyed Jafar Adnani Sadati, Email: jafaradnani@yahoo.com, , Email: jafaradnani1981@yahoo.com


Background: Cutaneous leishmaniasis (CL) remains a major health threatening disease in Iran and many countries around the world. Antimony compounds are currently used to treat CL. Due to the side effects and high resistance, the use of alternative therapies, especially the use of nanoparticles, has been considered by researchers. The aim of this study was to investigate the anti-leishmanial activity of selenium nanoparticles (SeNPs) on Leishmania major in vitro.

Methods: In this experimental study, the anti-leishmanial activity of the SeNPs was evaluated at concentrations of 1.25, 2.5, 5, 10, 25, 50, and 100 µg/mL at exposure times of 24, 48, and 72 hours on 106 live parasites. Then, the number of live parasites was counted by trypan blue using a neobar slide and light microscope (Hemocytometer method). Glucantime and distilled water were considered positive and negative controls, respectively. Then, 50% inhibitory concentration (IC50) values were calculated by SigmaPlot 13 software. All reactions were performed in triplicate, and the results were considered as average.

Results: The results of this study revealed that all concentrations of SeNPs have anti-leishmanial activity. The concentration of 100 µg/mL of SeNPs had the highest anti-leishmanial effect (100%) after 72 hours of exposure. Further, the IC50 content of SeNPs on L. major after 24, 48, and 72 hours was calculated to be 42.76, 34.53, and 22.69 µg/mL, respectively.

Conclusions: The results indicated that SeNPs in different concentrations has an inhibitory effect on the growth of L. major. However, further investigations are required to determine the efficacy of SeNPs in vivo.

Please cite this article as follows: Norouzi R, Mirzaei F, Siadatpanah A, Adnani Sadati SJ. Evaluation of anti-leishmanial effect of selenium nanoparticles on Leishmania major promastigotes In Vitro. Avicenna J Clin Microbiol Infect. 2022; 9(3):130-134. doi:10.34172/ajcmi.2022.3393
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