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Submitted: 28 Mar 2021
Accepted: 23 Jun 2021
ePublished: 29 Jun 2021
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Avicenna J Clin Microbiol Infect. 2021;8(2): 45-50.
doi: 10.34172/ajcmi.2021.09
  Abstract View: 830
  PDF Download: 882

Original Article

The Effect of Bacteriocin Isolated From Lactobacillus rhamnosus on Pseudomonas aeruginosa Lipopolysaccharides

Hafizeh Haghighatafshar 1 ORCID logo, Reza Talebi 2, Amir Tukmechi 3* ORCID logo

1 Department of Microbiology, Urmia Branch, Islamic Azad University, Urmia, Iran
2 Department of Microbiology, Faculty of Basic Science, Urmia Islamic Azad University, Urmia, Iran
3 Department of Pathobiology and Quality Control, Artemia and Aquatic Animals Research Institute, Urmia University, Urmia, Iran
*Corresponding Author: *Corresponding author: Amir Tukmechi, Department of Pathobiology and Quality Control, Artemia and Aquatic Animals Research Institute, Urmia University, Urmia, Iran. Tel: +989141473201, Email: , Email: atokmachi@gmail.com

Abstract

Background: Bacteriocins are heterogeneous inhibitory substances that could affect the bacteria belonging to the same genus. Both gram-positive and gram-negative bacteria produce bacteriocins. One of the best sources of producing bacteriocins is Lactobacillus. The aim of this study was to isolate and purify bacteriocin from Lactobacillus rhamnosus and assess its effects on Pseudomonas aeruginosa and synthesis of its lipopolysaccharide.

Methods: L. rhamnosus was prepared and cultured at MRS broth and incubated at 37ºC for 24 hours. Then, the medium was centrifuged for the isolation of bacteriocin and the supernatant was considered as bacteriocin. Antibacterial properties of different concentrations of bacteriocin (50, 100, 200, and 400 μg/mL) against P. aeruginosa were assayed by using agar diffusion and broth micro dilution methods. Also, the effect of bacteriocin against lipopolysaccharide synthesis in P. aeruginosa was analyzed by using one unit of minimum inhibitory concentration (MIC) for bacteriocin.

Results: The results showed that all bacteriocin concentrations had antibacterial activity against P. aeruginosa. The MIC value was 31.25 μg/mL and minimal bactericidal concentration (MBC) was 62.5 μg/mL. Also, the synthesis of lipopolysaccharide decreased during P. aeruginosa growth period, and it reached zero after 5 hours.

Conclusions: The results of this study showed the antibacterial effect of bacteriocin isolated from L. rhamnosus against P. aeruginosa. In addition, this bacteriocin prevented the lipopolysaccharide synthesis in P. aeruginosa.




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