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Submitted: 29 Oct 2016
Revision: 22 Feb 2017
Accepted: 29 Mar 2017
ePublished: 07 May 2017
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Avicenna J Clin Microbiol Infect. 2017;4(3): 43457.
doi: 10.5812/ajcmi.43457
  Abstract View: 1501
  PDF Download: 717

Research Article

Preliminary Evaluation of the Antimicrobial Activity of Total Extract and Fractions of Chloroform, Methanol, and Aqueous from the Aerial Parts of Salvia aegyptiaca

Mojdeh Hakemi-Vala 1*, Ayda Eslamzadeh 2, Fatemeh Bagheri Bejestani 2, Jinous Asgarpanah 2, Mohsen Heidary 3, Saeed Khoshnood 4

1 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
3 Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
4 Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
*Corresponding Author: Corresponding author: Mojdeh Hakemi-Vala, Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran, Email: m.hakemi@sbmu.ac.ir

Abstract

Background: Increased microbial resistance to conventional medicines and their side effects have led to studying the effect of herbal extracts on microorganisms.

Objectives: The current study aimed at evaluating the antimicrobial activity of total extract and fractions of chloroform, methanol, and aqueous of aerial parts of Salvia aegyptiaca.

Methods: The plants were collected from Bandar Abbas, Iran, in April 2015. The methanol extract of aerial parts, in addition to chloroform, methanol, and aqueous fractions, were prepared by the maceration method from S. aegyptiaca. The antimicrobial activity of fractions were determined by cup plate and further micro-dilution methods based on the Clinical and Laboratory Standards Institute (CLSI) 2013 against some Gram-positive and -negative bacteria as well as Candida albicans. Statistical analysis was conducted by t test.

Results: Significant results were obtained regarding the tested microorganisms only by micro-dilution method.

Conclusions: There were no significant results in plant extract and its fractions against the tested microorganisms in the cup plate method, which may be due to the inability of agar diffusion. In contrast, by excluding the agar’s hindrance property, verified results of micro-dilution method were deleted.


Copyright © 2017, Hamadan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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