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Submitted: 11 Jan 2015
Revised: 11 Mar 2015
Accepted: 15 Apr 2015
First published online: 01 Aug 2015
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Avicenna J Clin Microbiol Infect. 2015;2(3): e26905.
doi: 10.17795/ajcmi-26905
  Abstract View: 302
  PDF Download: 401

Research Article

Phenotypic and Genotypic Characteristics of Listeria monocytogenes Isolated From Dairy and Meat Products

Abbas Bahador 1, Behrooz Sadeghi Kalani 2, Firuzeh Valian 3, Gholamreza Irajian 2 * , Lida Lotfollahi 4 *

1 Department of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran
2 Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, IR Iran
3 Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IR Iran
4 Department of Microbiology, Faculty of Medicine, UrmiaUniversity of Medical Sciences, Urmia, IR Iran
Corresponding author: Lida Lotfollahi, Department of Microbiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, IR Iran. Tel: +98-9144470366, Fax: +98-2188058649, Email: liphd83@yahoo.com

Abstract

Background: Listeria monocytogenes is a foodborne pathogen and a serious threat to the public health in the world. Consumption of traditional foods such as dairy and meat products can be a major reason for relative abundance and isolation of these bacteria.

Objectives: The purpose of this study was to determine the phenotypic and genotypic characteristics of L. monocytogenes strains isolated from dairy and meat products.

Materials and Methods: A total of 317 dairy products and meat-processed samples were collected. Antibiotic susceptibility test was performed on each sample by the disk diffusion method (Kirby Bauer). Five reference loci were used for typing of L. monocytogenes strains by MLVA (Multiple Locus VNTR Analysis) Technique.

Results: A total of 24 L. monocytogenes isolates were collected from the dairy and meat products. Resistance of isolated L. monocytogenes strains to penicillin G were 54.54% (from dairy products) and 46.15% (from processed meat). Genetic relatedness of isolates were assessed by MLVA. Out of 13 different types, type 2 with 6 strains and type 3 with 4 strains, were the most common types.

Conclusions: MLVA analysis showed that samples obtained from different sources could have similar genetic profile. As a result, administration of penicillin in patients with listeriosis (especially pregnant women) and antibiotic susceptibility test are recommended. The fast and accurate methods such as MLVA for tracking of pollution sources of L. monocytogenes are recommended during outbreaks.

Keywords: Genotypic, Dairy Products, Meat, Listeria monocytogenes
Copyright © 2015, Hamadan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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