Hamadan University of Medical Sciences Avicenna Journal of Clinical Microbiology and Infection 2383-0298 13 3 2026 09 29 Enhanced Protoscolicidal Activity of Pulsed Electric Fields Combined With Albendazole: An in Vitro Study 174 180 10.34172/ajcmi.3800 EN Bahman Maleki https://orcid.org/0009-0009-2975-3771 Zeinab Shankayi Abolfazl Salehi Seyed Mahmoud Sadjjadi Hasanali Mohebbi Mohammad Saeed Tahmasbi Somar Tahereh Mohammadzadeh https://orcid.org/0000-0002-5427-9882 Journal Article 10.34172/ajcmi.3800 2025 09 21 2026 06 03 Introduction: Cystic echinococcosis (CE), caused by the metacestode stage of Echinococcus granulosus, remains a significant zoonotic disease with serious health and economic burden worldwide, especially in endemic regions. Despite the availability of surgical and pharmacological treatments, complications such as cyst content leakage and recurrence are prevalent, necessitating novel and more effective therapeutic approaches. Accordingly, this study aimed to evaluate the synergistic protoscolicidal effects of pulsed electric fields (PEF) and albendazole (ALB). Methods: Protoscoleces were aseptically extracted from hydatid cysts in sheep livers and exposed to PEF at intensities of 70, 150, and 1300 V/cm for 15–60 minutes. Experimental groups included (1) PEF alone, (2) PEF combined with ALB, and (3) ALB alone. Moreover, viability of protoscoleces was assessed using the standard eosin exclusion assay. Results: PEF alone increased mortality in a time-dependent and intensity-dependent manner, reaching 100% at 60 minutes. In addition, PEF+ALB (100 μg/mL) and ALB alone (100 μg/mL) achieved 100% mortality at 30–45 and 60 minutes, respectively. Nonetheless, PEF+ALB significantly outperformed PEF alone at early time points: 70 V/cm/15 minutes (72.03% vs. 87.6%, P<0.01), 1300 V/cm/15 minutes (86.79% vs. 95%, P<0.05), and 1300 V/cm/30 minutes (94.3% vs. 100%, P<0.01). Conclusion: While these findings provide preliminary in vitro proof-of-concept for a synergistic effect, clinical application remains premature. Thus, further investigations must prioritize experiments with clinically relevant ALB concentrations, ex vivo studies on intact cysts, and in vivo validation. The clinical applicability of PEF in CE management requires extensive further research.