Hamadan University of Medical Sciences Avicenna Journal of Clinical Microbiology and Infection 2383-0298 9 1 2022 03 29 Current Perspectives on Viable but Non-culturable Bacteria in Food Safety and Public Health 31 40 10.34172/ajcmi.2022.06 EN Sharareh Shamloei https://orcid.org/0000-0001-9912-6399 Ali Nabavi-Rad https://orcid.org/0000-0001-7799-9404 Habibollah Nazem Abbas Yadegar https://orcid.org/0000-0002-2135-7581 REVIEW 10.34172/ajcmi.2022.06 2022 02 26 2022 03 17 The viable but non-culturable (VBNC) state is defined as an adaptive mechanism for microorganisms adjusting to stressful conditions. Although VBNC bacteria are alive and metabolically active, they are unable to grow on routine culture media. Nevertheless, the potential capacity of VBNC pathogens to retain virulence activity and further resuscitate into the culturable state in favorable conditions constitutes a major hazard to food safety and public health. Food processing, transformation, and storage, as well as non-thermal techniques, can provoke pathogens toward VBNC induction. The distinct characteristic of VBNC bacteria led to the emergence of novel culture-independent techniques to prevent the misinterpretation of food safety. To deepen our knowledge of the molecular aspect of the VBNC state, several mechanism-oriented studies investigated the metabolic activity of VBNC bacteria and their correlation with different stressful conditions. This review aims to discuss the molecular mechanisms and genomic factors underlying the induction and resuscitation of the VBNC state. The study will further highlight innovative detection methods to provide a comprehensive perspective for future studies in the emerging fields of research concerning VBNC state, food safety, and public health.