Hamadan University of Medical Sciences Avicenna Journal of Clinical Microbiology and Infection 2383-0298 6 4 2019 12 31 Molecular Rapid Diagnostic Test for Nosocomial Nonfermenting Gram-Negative Bacilli 138 141 10.34172/ajcmi.2019.25 EN Amir Emami https://orcid.org/0000-0002-4510-1820 Neda Pirbonyeh Abdollah Bazargani Fatemeh Javanmardi Journal Article 10.34172/ajcmi.2019.25 2019 11 25 2019 12 08 Accurate diagnosis of nosocomial infections is crucial for appropriate antibiotic therapy, as well as avoiding unnecessary use of drugs. Mainly 3 gram-negative bacteria including Pseudomonas aeruginosa, Acinetobacter baumannii, and Stenotrophomonas maltophilia are involved in nosocomial infections. These bacteria are possibly misdiagnosed with together in traditional diagnostic methods. In this regard, a rapid molecular diagnostic kit was developed in Shiraz Burn Research Center. Using this diagnostic kit, the sensitivity and specificity analyses showed following results, respectively: P. aeruginosa: 100%, 96.8%; A. baumannii: 100%, 100%, and S. maltophilia: 96.7%, and 93.8%. Therefore, this multiplex polymerase chain reaction (PCR) method with 3 primer sequences could be a responsive technique in timely and appropriate detection of studied bacteria.