Hamadan University of Medical Sciences Avicenna Journal of Clinical Microbiology and Infection 2383-0298 5 1 2018 02 01 The Prevalence of Panton-Valentine Lukocidin Gene in Staphylococcus aureus Species Isolated From Nosocomial Infections in Isfahan, Iran 60088 60088 10.5812/ajcmi.60088 EN Fahimeh Nourbakhsh https://orcid.org/0000-0002-5120-560X Samaneh Borooni Elaheh Tajbakhsh Journal Article 10.5812/ajcmi.60088 2017 08 12 2018 01 07 Background: Staphylococcus aureus is a significant pathogen and major cause of nosocomial and community-acquired infections. The current study aimed at investigating the frequency of Panton-Valentine leukocidin (PVL) gene as a serious virulence factor causing WBC destruction. Methods: Collectively, 100 species of S. aureus were isolated from Isfahan, Iran, hospitals and confirmed by biochemical tests (coagulase, mannitol fermentation, and DNase). The antibiotic resistance patterns were studied by the disk diffusion method. Results: Out of the 100 isolates, 56.2% were PVL positive of which 19.8% from abscess, 51.7% from wound, 23.2% from bedsore, and 5.3% from tracheal secretion. Among the detected isolates, 87.8% were resistant to methicillin. Conclusions: The current study showed the high frequency of PVL in wound strains. Further studies are required to understand the distribution of these virulent isolates in order to decrease the risk of infection. High quality hospital cares as well as new antibiotics is required to combat the multidrug resistant bacteria.