Hamadan University of Medical Sciences Avicenna Journal of Clinical Microbiology and Infection 2383-0298 5 3 2018 08 01 Characterization of Staphylococcus aureus Isolated From Wound Infectious in Diabetes Clinic of Hazrat Fatemeh Zahra (SA) Hospital 67 70 10.34172/ajcmi.2018.13 EN Vajiheh Nourbakhsh https://orcid.org/0000-0002-5257-9443 Fahimeh Nourbakhsh https://orcid.org/0000-0001-5576-7274 Elaheh Tajbakhsh Samaneh Borooni Dana Daneshmand Journal Article 10.34172/ajcmi.2018.13 2018 07 12 2018 08 05 Background: Staphylococcus aureus is a gram positive pathogen which causes a wide range of infections. The present study aimed to investigate genotypic and phenotypic screening about biofilm formation in S. aureus isolated from wound infections in a diabetes clinic in Hazrat Fatemeh Zahra (SA) hospital. Methods: A total of 267 clinical samples were collected from various types of wound infections in the diabetes clinic of Hazrat Fatemeh Zahra (SA) hospital, Isfahan, Iran. The methicillin-resistant S. aureus (MRSA) isolates were selected and biofilm formation and its related genes were analyzed by polymerase chain reaction (PCR). Results: The results showed that 95 out of 132 samples were MRSA. The high resistance was seen to methicillin, erythromycin, ciprofloxacin, and penicillin. Phenotypic results showed that 48.3% of the isolates were high biofilm producers, 29.1% were average biofilm producers, and 10.6% were low biofilm producers. Conclusions: According to the results of this study, the expression levels of biofilm-associated genes significantly increased, and high prevalence of antibiotic resistance was one of the important reasons for the development of drug resistance in patients.