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Submitted: 21 Mar 2016
Revision: 20 Apr 2016
Accepted: 13 May 2016
ePublished: 15 Jun 2016
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Avicenna J Clin Microbiol Infect. 2016;3(3): 37480.
doi: 10.17795/ajcmi-37480
  Abstract View: 1437
  PDF Download: 2368

Research Article

Identification of Pathogenic Bacteria in Blood Cultures and Susceptibility Testing of Isolates With Various Antibiotics

Mehdi Kholoujini 1, Pezhman Karami 2, Azad Khaledi 3, Alireza Neshani 3, Parastoo Matin 4, Mohmad Yosef Alikhani 2*

1 Faculty of Medicine, Tarbiat Modares University, Tehran, IR Iran
2 Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran
3 Antimicrobial Resistance Research Center, Avicenna Research Institute, Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran
4 Microbiology Laboratory of Shahid Beheshti Hospital, Hamadan University of Medical Sciences, Hamadan , IR Iran
*Corresponding Author: Corresponding author: Mohmad Yosef Alikhani, Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran. Tel: +98-9125443147, Email: alikhani43@yahoo.com

Abstract

Background: Blood infections are an extensive range of disorders that can vary from limited bacteremia to fatal septicemia. Bacteremia refers to the transient presence of a bacterium in the bloodstream. A delay in the diagnosis and treatment of sepsis can cause mortality, with a 20% - 50% prevalence rate.

Objectives: Due to the changing patterns of antibiotic resistance, as well as differences in patterns over time in different settings, we decided to identify infectious agents and their antibiotic resistance patterns in blood cultures.

Materials and Methods: This study was conducted at Shahid Beheshti hospital, Hamadan, Iran, during a one-year period (March 21, 2014, to March 22, 2015). From patients with suspected bloodstream infections, 5-10 mL of blood was collected three times and inoculated into culture bottles. After identifying the types of microorganisms, susceptibility testing was performed according to CLSI standards, and the results were analyzed with statistical software.

Results: In the present study, 2,130 blood cultures were obtained from 710 patients (384 females and 326 males). Of these cultures, 232 (18.9%) were positive; 107 (46%) and 125 (54%) were from females and males, respectively. Most of the positive cultures were related to the internalmedicine and hematology wards, which had 132 cases (56.9%), and the ICU, with 37 cases (16%). Themost frequent isolates were Pseudomonas aeruginosa, Escherichia coli, Acinetobacter baumannii, and coagulase-negative Staphylococcus aureus, with prevalence rates of 18.2%, 24.1%, 10.3%, and 10.3%, respectively. The most effective antibiotic against Gram-positive isolates was vancomycin.

Conclusions: This study revealed that the most effective antibiotics against two Gram-negative and Gram-positive groups were amikacin and norfloxacin, so it is recommended that these antibiotics be used empirically, at least in the setting where this study was conducted, before performing the culturing and antibiogram process.


Copyright © 2016, Hamadan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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