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Submitted: 11 Nov 2015
Accepted: 18 Dec 2015
ePublished: 30 Jan 2016
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Avicenna J Clin Microbiol Infect. 2016;3(1): 34575.
doi: 10.17795/ajcmi-34575
  Abstract View: 1542
  PDF Download: 915

Research Article

Antioxidant Effect of Lactobacillus acidophilus as a Probiotic at Different Time Intervals

Fermix Nazari 1, Yegane Delborde 1, Zahra Karimitabar 2, Akram Ranjbar 3*, Mohammad Yosef Alikhani 2,4*

1 Students Research Center, Hamadan University of Medical Sciences, Hamadan, IR Iran
2 Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran
3 Department of Toxicology and Pharmacology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, IR Iran
4 Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, IR Iran
*Corresponding Authors: Corresponding author:Akram Ranjbar, Department of Toxicology and Pharmacology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, IR Iran. Tel/Fax: +98-8138380551,, Email: akranjbar2015@gmail.com; Corresponding author: Mohammad Yosef Alikhani, Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran, Email: alikhani43@yahoo.com

Abstract

Background: Probiotics are survival microorganisms that, when administered in sufficient amounts, confer health benefits to the host and can be used in an antioxidative role.

Objectives: The antioxidative effect of whole cells and intracellular cell-free extracts of the lactic acid bacteria Lactobacillus acidophilus (PTCC 1643) as a probiotic at three different time intervals was investigated.

Materials and Methods: Antioxidant biomarkers, such as total antioxidant power (TAP), measured with the FRAP (ferric-reducing ability of plasma) method, were evaluated at 24, 48, and 72 hours.

Results: The results showed that extracts and bacteria of L. acidophilus were able to significantly increase TAP after 24 and 72 hours.

Conclusions: The results showed that the effect of L. acidophilus is time-dependent.


Copyright © 2016, Hamadan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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