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Submitted: 18 May 2015
Revision: 08 Oct 2015
Accepted: 18 Oct 2015
ePublished: 23 Nov 2015
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Avicenna J Clin Microbiol Infect. 2015;2(4): 29791.
doi: 10.17795/ajcmi-29791
  Abstract View: 1736
  PDF Download: 718

Research Article

Campylobacter jejuni and Campylobacter coli in Children With Acute Diarrhea in Health Centers of Hamadan, Iran

Sahar Rastyani 1, Mohammad Yousef Alikhani 1,2, Iraj Sedighi 3, Sima Kazemi 1, Hamed Farhadi Kohan 1, Mohammad Reza Arabestani 1,2*

1 Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran
2 Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, IR Iran
3 Department of Pediatric, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran
*Corresponding Author: Corresponding author: Mohammad Reza Arabestani, Department of Microbiology, Faculty of medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran. Tel: +98-9188662009, Fax: +98-8138380130, Email: mohammad.arabestani@gmail.com

Abstract

Background: Enteritis caused by Campylobacter is considered as the most common acute bacterial diarrhea around the world. In most cases, infection occurs as a result of consuming contaminated water or food, especially raw meat of fowls.

Objectives: The purpose of the present study was to determine the prevalence and antibiotic resistance of campylobacter species among pediatrics of Hamadan city, Iran.

Patients and Methods: A total of 120 stool samples from children less than 10 years old were examined from January 2013 to December 2014 in Hamadan, Iran. The samples were incubated in Campy-Thio enrichment medium for 1 - 2 hours and then cultured on a specific medium; after that, the suspected colonies were analyzed for Campylobacter spp. identification by conventional tests. The identified species by biochemical methods were confirmed by polymerase chain reaction (PCR). Antimicrobial susceptibility testing was performed by disk agar diffusion (DAD) method.

Results: Twelve (10%) Campylobacter spp. from 120 stool samples were isolated including C. coli and C. jejuni. In the antibiotic susceptibility test, the most frequent resistance was observed to ciprofloxacin 8 (88.8%), followed by 7 (77.7%) resistant strains to tetracycline, 7 (77.7%) to erythromycin, 6 (66.6%) to clindamycin, 5 (55.5%) to meropenem, 4 (44.4%) to gentamicin, 3 (33.3%) to nalidixicacid and only 1 (11.1%) to chloramphenicol.

Conclusions: Campylobacter is responsible for some important clinical problems such as enteritis and is also associated with meningitis and hemolytic-uremic syndrome. It is imperative to monitor the prevalence and antibiotic resistance of Campylobacter spp. as well as other the zoonotic bacteria.


Copyright © 2015, Hamadan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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