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Submitted: 07 Jul 2023
Revision: 17 Jul 2023
Accepted: 28 Jul 2023
ePublished: 29 Sep 2023
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Avicenna J Clin Microbiol Infect. 2023;10(3): 95-99.
doi: 10.34172/ajcmi.3471
  Abstract View: 187
  PDF Download: 99

Original Article

Genotypic Investigation of Antibiotic Resistant blaOXA-4 Gene in Clinical Isolates of Pseudomonas aeruginosa

Milad Gholampour Matin 1 ORCID logo, Reza Shapouri 1, Mohammadreza Nahaei 2* ORCID logo, Mojtaba Mohammadi Roknabadi 1, Rasoul Shokri 1

1 Department of Microbiology, Faculty of Basic Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran
2 Department of Microbiology and Laboratory Sciences, Faculty of Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran
*Corresponding Author: Mohammadreza Nahaei, Email: nahaeim@yahoo.com

Abstract

Background: Pseudomonas aeruginosa, an opportunistic Gram-negative bacterium, is responsible for 10-15% of hospital infections worldwide. The acquisition of resistance genes is one of the important mechanisms that causes the spread of resistance in this bacterium. This study aimed to conduct a phenotypic and genotypic investigation of the blaOXA-4 resistance gene in P. aeruginosa isolated from clinical samples.

Methods: In this study, 110 P. aeruginosa strains were isolated from various clinical samples. The disk diffusion method was applied to reveal the resistance pattern in the isolates. Moreover, the combined disk method was used for the phenotypic analysis of extended-spectrum beta-lactamases (ESBL). Finally, the presence of the blaOXA-4 beta-lactamase gene was analyzed genotypically by polymerase chain reactions (PCR) method.

Results: The highest sensitivity and resistance of the isolates were related to amikacin (65.45%) and ceftazidime (86.36%), respectively. The phenotypic analysis indicated that 72 isolates (65.45%) of P. aeruginosa are ESBL-producing. Furthermore, the presence of blaOXA-4 was approved genotypically in 33 P. aeruginosa isolates (45.83%).

Conclusion: This study revealed a high prevalence of antibiotic-resistant isolates of P. aeruginosa in the East Azerbaijan population that may be associated with the presence of the blaOXA-4 gene. However, further studies are necessary to identify other resistant genes in ESBL-producing isolates and other geographical areas with larger sample size.


Please cite this article as follows: Gholampour Matin M, Shapouri R, Nahaei M, Mohammadi Roknabadi M, Shokri R. Genotypic investigation of antibiotic resistant blaOXA-4 gene in clinical isolates of Pseudomonas aeruginosa. Avicenna J Clin Microbiol Infect. 2023; 10(3):95-99. doi:10.34172/ajcmi.3471
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