Amir Emami
1* 
, Neda Pirbonyeh
2, Abdollah Bazargani
3, Fatemeh Javanmardi
41 Assistant Professor of Microbiology (Ph.D.), Burn and Wound Healing Research Center, Microbiology Department, Shiraz University of Medical Sciences, Shiraz, Iran
2 M.Sc. in Microbiology, Burn and Wound Healing Research Center, Microbiology Department, Shiraz University of Medical Sciences, Shiraz, Iran
3 Associate Professor of Microbiology, Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Science, Shiraz, Iran
4 M.Sc. in Biostatistics, Burn and Wound Healing Research Center, Microbiology Department, Shiraz University of Medical Sciences, Shiraz, Iran
*Corresponding Author: *Corresponding author: Dr. Amir Emami, Ph.D., Assistant Professor of Microbiology, Burn and Wound Healing Research Center, Microbiology department, Amir-Al- Momenin Burn Hospital, Sadra Blv., Shiraz, Fars, Iran Phone: +98917(316)8640 Email: emami_a@sums.ac.ir; , Email:
emami.microbia@gmail.com
Abstract
Accurate diagnosis of nosocomial infections is crucial for appropriate antibiotic therapy, as well as avoiding unnecessary use of drugs. Mainly 3 gram-negative bacteria including Pseudomonas aeruginosa, Acinetobacter baumannii, and Stenotrophomonas maltophilia are involved in nosocomial infections. These bacteria are possibly misdiagnosed with together in traditional diagnostic methods. In this regard, a rapid molecular diagnostic kit was developed in Shiraz Burn Research Center. Using this diagnostic kit, the sensitivity and specificity analyses showed following results, respectively: P. aeruginosa: 100%, 96.8%; A. baumannii: 100%, 100%, and S. maltophilia: 96.7%, and 93.8%. Therefore, this multiplex polymerase chain reaction (PCR) method with 3 primer sequences could be a responsive technique in timely and appropriate detection of studied bacteria.