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Submitted: 25 Nov 2019
Accepted: 08 Dec 2019
ePublished: 31 Dec 2019
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Avicenna J Clin Microbiol Infect. 2019;6(4): 138-141.
doi: 10.34172/ajcmi.2019.25
  Abstract View: 670
  PDF Download: 506
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Brief Report

Molecular Rapid Diagnostic Test for Nosocomial Nonfermenting Gram-Negative Bacilli

Amir Emami 1* ORCID logo, Neda Pirbonyeh 2, Abdollah Bazargani 3, Fatemeh Javanmardi 4

1 Assistant Professor of Microbiology (Ph.D.), Burn and Wound Healing Research Center, Microbiology Department, Shiraz University of Medical Sciences, Shiraz, Iran
2 M.Sc. in Microbiology, Burn and Wound Healing Research Center, Microbiology Department, Shiraz University of Medical Sciences, Shiraz, Iran
3 Associate Professor of Microbiology, Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Science, Shiraz, Iran
4 M.Sc. in Biostatistics, Burn and Wound Healing Research Center, Microbiology Department, Shiraz University of Medical Sciences, Shiraz, Iran
*Corresponding Author: *Corresponding author: Dr. Amir Emami, Ph.D., Assistant Professor of Microbiology, Burn and Wound Healing Research Center, Microbiology department, Amir-Al- Momenin Burn Hospital, Sadra Blv., Shiraz, Fars, Iran Phone: +98917(316)8640 Email: emami_a@sums.ac.ir; , Email: emami.microbia@gmail.com

Abstract

Accurate diagnosis of nosocomial infections is crucial for appropriate antibiotic therapy, as well as avoiding unnecessary use of drugs. Mainly 3 gram-negative bacteria including Pseudomonas aeruginosa, Acinetobacter baumannii, and Stenotrophomonas maltophilia are involved in nosocomial infections. These bacteria are possibly misdiagnosed with together in traditional diagnostic methods. In this regard, a rapid molecular diagnostic kit was developed in Shiraz Burn Research Center. Using this diagnostic kit, the sensitivity and specificity analyses showed following results, respectively: P. aeruginosa: 100%, 96.8%; A. baumannii: 100%, 100%, and S. maltophilia: 96.7%, and 93.8%. Therefore, this multiplex polymerase chain reaction (PCR) method with 3 primer sequences could be a responsive technique in timely and appropriate detection of studied bacteria.
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