Abstract
Background: Pseudomonas aeruginosa is a common opportunistic pathogen that causes nosocomial
infection in immunocompromised patients. Among different virulence factors, the type III secretion system
(TTSS) is an important agent in virulence and development of antimicrobial resistance in P. aeruginosa.
Previous studies have shown that production of TTSS proteins was correlated with increasing virulence
and resistance to several antibiotics. In this study, the exotoxins genes (exoU and exoS) and pattern of
antimicrobial susceptibility in clinical P. aeruginosa isolates were determined.
Methods: A total of 175 P. aeruginosa isolates were collected from patients hospitalized in Shahrekord
and Chamran educational hospitals of Isfahan, Iran (during April to December 2015). Antimicrobial
susceptibility test was performed by disk diffusion test. The presence of exotoxin genes was detected using
multiplex polymerase chain reaction (PCR) of exoU and exoS genes.
Results: The antibiotic resistance rate was higher than 70% to many antibiotics. The highest rates of
resistance (155 and 148) were related to Levofloxacin (88.6%) and Meropenem (84.6%), respectively.
The exoU gene was found in 75 (42.9%) isolates and 136 (77.7%) isolates carried the exoS. In addition, 36
(20.6%) of the isolates carried both genes. A statistical significance was observed between the presence of
exoU gene and resistance to piperacillin (P = 0.01).
Conclusions: The result of this study showed a high resistance rate to the most antibiotic classes and a
specific relationship between the virulence genotype and antimicrobial resistance especially more
virulent genotype of exoU+ . In order to prevent the spread of more virulent strains in health care facilities,
molecular methods alongside antimicrobial susceptibility tests are suggested.