Submitted: 30 May 2017
Revision: 03 Dec 2017
Accepted: 04 Jan 2018
ePublished: 10 Feb 2018
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)

Avicenna J Clin Microbiol Infect. 2018;5(1): 14249.
doi: 10.5812/ajcmi.14249
  Abstract View: 1182
  PDF Download: 1340

Research Article

Detection of Toxic Shock Syndrome Toxin (tsst) Gene Among Staphylococcus aureus Isolated from Patients and Healthy Carriers

Reza Hakimi Alni 1, Abdolmajid Mohammadzadeh 1* ORCID logo, Pezhman Mahmoodi 1, Mohammad Yousef Alikhani 2

1 Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamadan, Iran
2 Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
*Corresponding Author: Corresponding author: Abdolmajid Mohammadzadeh, Bu-Ali Sina University, Hamadan, Iran. Tel: +98-8134227350, Fax: +98-8134227475, Email: mohammadzadeh4@gmail.com


Background: Staphylococcus aureus is the major causative agent of hospital-acquired and community-acquired infections. These bacteria produce a wide variety of exotoxins, including Toxic Shock Syndrome Toxin (TSST) and virulence factors, which are thought to contribute to its pathogenic potential.

Objectives: The aim of this study was to identify tsst gene in S. aureus isolated from patients and healthy carriers.

Methods: In this cross-sectional study, a total of 60 humanS. aureus isolates were collected from individuals referred to Shahid Beheshti hospital (patients, n = 40) and healthy farm workers (n = 20) in Hamadan province of Iran. Thereafter, DNA samples were extracted using the phenol-chloroform method and the samples were investigated for tsst gene using a specific PCR assay.

Results: The DNA fragment corresponding to the tsst gene (326 bp) was observed in 45% (9 out of 20) of S. aureus isolated from healthy farm workers; while, 22.5% (9 out of 40) of patients’ isolates were found to be positive for tsst gene, which indicated that in total 30% of the isolates possessed this gene.

Conclusions: The results of the present study showed the high prevalence of the tsst gene among S. aureus isolated from healthy farm workers and patients. Therefore, appropriate precautions must be considered to decrease the risk of transmission of such isolates to other humans. 

Copyright © 2018, Avicenna Journal of Clinical Microbiology and Infection. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
First Name
Last Name
Email Address
Security code

Abstract View: 1183

Your browser does not support the canvas element.

PDF Download: 1340

Your browser does not support the canvas element.