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Submitted: 24 Apr 2017
Revision: 05 Nov 2017
Accepted: 23 Nov 2017
ePublished: 30 Nov 2017
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Avicenna J Clin Microbiol Infect. 2017;4(4): 12086.
doi: 10.5812/ajcmi.12086
  Abstract View: 755
  PDF Download: 540

Research Article

Prevalence of Antibiotic Resistance and Class 1 Integrons in Clinical and Environmental Isolates of Pseudomonas aeruginosa

Mohammad Yousef Alikhani 1,2*, Siamak Parsavash 2, Mohammad Reza Arabestani 1, Seyed Mostafa Hosseini 2

1 Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, IR Iran
2 Microbiology Department, Hamadan University of Medical Sciences, Hamadan, IR Iran
*Corresponding Author: Corresponding author: Mohammad Yousef Alikhani, Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, IR Iran. Tel: +98-8138380130, Fax: +98-8138380130,, Email: alikhani43@yahoo.com

Abstract

Background: Many clinical isolates of Pseudomonas aeruginosa exhibit antibiotic-resistance and it is the main cause of hospital infections.

Objectives: The current study aimed at evaluating the diversity of genes coding for antibiotic resistance in clinical and environmental isolates of P. aeruginosa in Hamadan, West of Iran.

Methods: In the current cross sectional study, 100 clinical and 50 environmental isolates of P. aeruginosa were collected from October 2013 to May 2014. The standard disk diffusion method (SDD) was performed to test antibiotic susceptibility. All isolates were evaluated by the polymerase chain reaction (PCR) for the presence of class 1 integrons and genes encoding resistance to aminoglycoside antibiotics. The relationship between antibiotic resistance and integrons was tested by the 2-tailed chi-square (χ 2 ) or the Fisher exact tests. P values ≤ 0.05 were considered statistically significant.

Results: The most common antibiotic resistance was to cotrimoxazole (61.3%). The least observed resistance was to meropenem (9.3%) and piperacillin /tazobactam (12%). The result of minimum inhibitory concentration (MIC) for amikacin showed that 20% of the strains were resistant, 15% had intermediate resistance, and 65% were sensitive. Class 1 integrons were found in 57% of the isolates. The aminoglycoside-resistance cassette included aadA (confers resistance to streptomycin and spectinomycin) and aphA1 (encoded aminoglycoside 3’-phosphotransferase) genes were the most prevalent in the isolates.

Conclusions: Antibiotic resistance had a high prevalence in clinical isolates of P. aeruginosa and was commonly associated with class 1 integrons. The knowledge of drug resistance patterns helps to apply effective antibiotic treatments and appropriate infection control measures to prevent the spread of infection in hospitals.


Copyright © 2017, Avicenna Journal of Clinical Microbiology and Infection. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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