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Submitted: 30 Jan 2017
Revision: 02 Mar 2017
Accepted: 29 Mar 2017
ePublished: 23 Apr 2017
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Avicenna J Clin Microbiol Infect. 2017;4(2): 46049.
doi: 10.5812/ajcmi.46049
  Abstract View: 1612
  PDF Download: 757

Research Article

Genotype Diversity and Evaluation of Biofilm Formation in sasX Positive Methicillin-Resistant Staphylococcus aureus

Morteza Eshaghi 1, Meysam Hasannejad Bibalan 1, Mahdi Rohani 2, Manzar Sadat Ghaffari 3, Malihe Talebi 1*

1 Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran. IR Iran
2 Department of Microbiology, Pasteur Institute of Iran, Tehran, IR Iran
3 Health Center Office of Delijan, Arak University of Medical Sciences, Arak, IR Iran
*Corresponding Author: Corresponding author: Malihe Talebi, Associate Professor of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Postal code: 1384987154, Tehran, IR Iran. Tel/Fax: +98-21880586491, Email: talebi.m@iums.ac.ir

Abstract

Objectives: Staphylococcus aureus surface protein X (sasX) is a newly described protein that has different roles in virulence, such as biofilm formatin, as a bacterial approach for pathogenesis. SasX gene was initially found in eastern Asian countries, yet, studiese have shown spreading of this gene to other strains. Because there is no report on this gene in Iran, the current study aimed at determining the prevalence, genotype diversity, and evaluation of biofilm formation in sasX positive methicillin-resistant Staphylococcus aureus (MRSA).

Methods: This experimental study was conducted on strains isolated from inpatients during 2014 to 2015. Cultivation, identification, and confirmation of MRSA isolates were performed. The evaluation of biofilm production, sasX gene detection, and Random amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) were done. Data collection and comparison were performed by SPSS and gelcompare softwares.

Results: Overall, 140 isolates (77.8%) formed a biofilm and 23 (12.6%) isolates were sasX positive. Amongst 23 sasX+ strains, 21 (91.3%) isolates created a biofilm. The RAPD-PCR analysis of 23 sasX positive isolates showed 19 RAPD types with 4 common types (CT) and 15 single types (ST).

Conclusions: Frequency of sasX gene was somewhat similar to another study in eastern countries and the ability of biofilm formation in sasX+ strains compared to all of MRSA isolates were not significant (P value > 0.05) and a correlation was not found between RAPD type and biofilm grade.


Copyright © 2017, Hamadan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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