Background: Staphylococcus aureus is a gram positive pathogen which causes a wide range of infections. The
present study aimed to investigate genotypic and phenotypic screening about biofilm formation in S. aureus
isolated from wound infections in a diabetes clinic in Hazrat Fatemeh Zahra (SA) hospital.
Methods: A total of 267 clinical samples were collected from various types of wound infections in the diabetes
clinic of Hazrat Fatemeh Zahra (SA) hospital, Isfahan, Iran. The methicillin-resistant S. aureus (MRSA) isolates
were selected and biofilm formation and its related genes were analyzed by polymerase chain reaction (PCR).
Results: The results showed that 95 out of 132 samples were MRSA. The high resistance was seen to methicillin,
erythromycin, ciprofloxacin, and penicillin. Phenotypic results showed that 48.3% of the isolates were high
biofilm producers, 29.1% were average biofilm producers, and 10.6% were low biofilm producers.
Conclusions: According to the results of this study, the expression levels of biofilm-associated genes significantly
increased, and high prevalence of antibiotic resistance was one of the important reasons for the development
of drug resistance in patients.