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Submitted: 12 Sep 2016
Revision: 18 Dec 2016
Accepted: 27 Dec 2016
ePublished: 23 Apr 2017
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Avicenna J Clin Microbiol Infect. 2017;4(2): 42244.
doi: 10.5812/ajcmi.42244
  Abstract View: 1106
  PDF Download: 790

Research Article

Staphylococcal Cassette Chromosome mec (SCCmec) Typing of Methicillin-Resistant Staphylococcus aureus Strains Isolated from Community- and Hospital-Acquired Infections

Fahimeh Ghanbari 1, Shirin Saberianpour 2, Fatemeh-sadat Zarkesh-Esfahani 3, Nafiseh Ghanbari 4, Azadeh Taraghian 5, Farzad Khademi 6*

1 Student Research Committee, School of Medicine, Shahid Saddoghi University of Medical Sciences, Yazd, Iran
2 Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
3 Department of Microbiology, Faculty of Bioscience, Falavarjan Branch Islamic Azad University, Falavarjan, Isfahan, Iran
4 Department of Biology, Science and Art University, Yazd, Iran
5 Department of Microbiology, School of Medicine, Esfahan University of Medical Sciences, Esfahan, Iran
6 Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
*Corresponding Author: Corresponding author: Farzad Khademi, Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran. Tel: +98-9149679332,, Email: k_farzad@yahoo.com

Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for the growing number of hospital- and community-acquired infections.

Objectives: In this study, we aimed to determine the prevalence of MRSA, its antimicrobial resistance profile, and molecular typing of strains isolated from different infections in Iran.

Methods: A total of 100S. aureus strains were isolated from various clinical specimens from Al-Zahra Hospital of Isfahan, Iran during January-June, 2015. Antimicrobial susceptibility test was performed, using the disk diffusion method. For identifying the MRSA phenotype, oxacillin agar screening was performed. Detection of mecA gene among the isolates was performed via polymerase chain reaction (PCR) using specific primers, followed by multiplex PCR for SCCmec typing of MRSA isolates.

Results: In the present study, 54 (54%) isolates were identified as MRSA. Overall, 12 (22.23%) and 42 (77.7%) isolates were obtained from community- and hospital-acquired infections, respectively. SCCmec typing among MRSA isolates showed that 19 (35.18%), 13 (24.07%), 6 (11.11%), 5 (9.25%), and 3 (5.55%) isolates contained SCCmec type III, type I, type IV, type II, and type V, respectively; however, 8 (14.81%) isolates were nontypable.

Conclusions: In the current study, SCCmec type III isolates were the most common among 54 MRSA isolates in a teaching hospital in the center of Iran. This finding might be attributed to antibiotic pressure, facilitating clonal selection.


Copyright © 2017, Hamadan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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