Background: Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for the growing number of hospital- and
Objectives: In this study, we aimed to determine the prevalence of MRSA, its antimicrobial resistance profile, and molecular typing
of strains isolated from different infections in Iran.
Methods: A total of 100S. aureus strains were isolated from various clinical specimens from Al-Zahra Hospital of Isfahan, Iran during
January-June, 2015. Antimicrobial susceptibility test was performed, using the disk diffusion method. For identifying the MRSA
phenotype, oxacillin agar screening was performed. Detection of mecA gene among the isolates was performed via polymerase
chain reaction (PCR) using specific primers, followed by multiplex PCR for SCCmec typing of MRSA isolates.
Results: In the present study, 54 (54%) isolates were identified as MRSA. Overall, 12 (22.23%) and 42 (77.7%) isolates were obtained from
community- and hospital-acquired infections, respectively. SCCmec typing among MRSA isolates showed that 19 (35.18%), 13 (24.07%),
6 (11.11%), 5 (9.25%), and 3 (5.55%) isolates contained SCCmec type III, type I, type IV, type II, and type V, respectively; however, 8 (14.81%)
isolates were nontypable.
Conclusions: In the current study, SCCmec type III isolates were the most common among 54 MRSA isolates in a teaching hospital
in the center of Iran. This finding might be attributed to antibiotic pressure, facilitating clonal selection.