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Submitted: 11 Jun 2016
Revision: 15 Aug 2016
Accepted: 28 Aug 2016
ePublished: 05 Sep 2016
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Avicenna J Clin Microbiol Infect. 2017;4(1): 39785.
doi: 10.17795/ajcmi-39785
  Abstract View: 323
  PDF Download: 212

Research Article

The Prevalence of acrA and acrB Genes Among Multiple-Drug Resistant Uropathogenic Escherichia coli Isolated From Patients With UTI in Milad Hospital, Tehran

Davood Maleki 1, Sahar Honarmand Jahromy 1 * , Shohreh Zare Karizi 1, Parisa Eslami 2

1 Department of Microbiology, Islamic Azad University, Varamin-Pishva Branch, IR Iran
2 Department of Microbiology, Central laboratory, Milad Hospital, Tehran, IR Iran
Corresponding author: Sahar Honarmand Jahromy, Department of Microbiology, Islamic Azad University, Varamin-Pishva Branch, IR Iran sahar_hj2@yahoo.com

Abstract

Background: Urinary tract infection (UTI) is one of the most common infectious diseases and nosocomial infections worldwide, and uropathogenic Escherichia coli is the primary cause of UTI. Due to increased antibiotic resistance and the emergence of multidrug resistant (MDR) UPEC clones, the treatment of UTI is difficult. The occurrence of MDR in E. coli has been attributed to the AcrAB-TolC complex of efflux pumps.

Objectives: The aim of this study was to complete a frequency evaluation of acrA and acrB genes among UPEC MDR strains isolated from patients with UTI who were admitted to Milad hospital in Tehran.

Methods: For 123 UPEC strains that were isolated and diagnosed from the urine samples of patients using biochemical tests, antibiotic susceptibility was carried out using the disc diffusion method according to CLSI guidelines. Isolates that were resistant to at least one antimicrobial agent in three or more of the categories were considered to be MDR. The presence and frequency of acrA and acrB genes was determined using PCR.

Results: The rates of antibiotic resistance to ampicillin, cefalotin, tetracycline, cefazolin, ceftriaxone, ceftizoxime, ceftazidime, ciprofloxacin, and cotrimoxazole were 82.9%, 78.1%, 61.1%, 49.5%, 38.2%, 30.2%, 26.1%, 42.2%, and 60.1%, respectively. The isolates were most sensitive to nitrofurantoin (95.9%), gentamicin (77.2%), and amikacin (71.5%). A total of 78% of the isolates were MDR. The frequency of the acrAgene was 82.90%, the acrBgene was 95.90% andacrA+acrBwas 95.90%. There was no significant difference between acrA and acrB frequency relating to bacterial antibiotic resistance.

Conclusions: Our results showed that ways to control the treatment of UTI for the prevention of MDR occurrence should be sought. For a better study of efflux pumps, a comprehensive and detailed study regarding the presence of efflux pumps gees is required.

Keywords: Uropathogenic Escherichia coli, Multi-Drug Resistant, acrA and acrB
Copyright © 2016, Hamadan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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