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Submitted: 24 Jan 2016
Revision: 18 Feb 2016
Accepted: 26 Feb 2016
ePublished: 19 May 2016
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Avicenna J Clin Microbiol Infect. 2016;3(2): 36521.
doi: 10.17795/ajcmi-36521
  Abstract View: 344
  PDF Download: 238

Research Article

Prevalence of A2143G and A2144G Point Mutations Responsible for Clarithromycin Resistance among Helicobacter pylori Strains in Bushehr, Iran

Saeed Tajbakhsh 1,2 * , Jamal Falahi 1, Niloofar Motamed 3, Seyed Masoud Tabib 4, Abbas Bahador 5, Somayyeh Gharibi 2

1 Department of Microbiology and Parasitology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, IR Iran
2 The Persian Gulf Tropical Medicine Research Center, Bushehr University of Medical Sciences, Bushehr, IR Iran
3 Department of Community Medicine, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, IR Iran
4 Department of Internal Medicine, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, IR Iran
5 Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran
Corresponding author: Saeed Tajbakhsh, Department of Microbiology and Parasitology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, IR Iran. Tel: +98-9177746164, Fax: +98-7733320657, tajbakhshsaeed@yahoo.com

Abstract

Background: Resistance to clarithromycin in Helicobacter pylori has become one of the most important reasons for failure of antibiotic eradication therapies. This resistance is predominantly caused by point mutations in the peptidyl transferase region of 23S rRNA.

Objectives: The aim of this study was to determine the prevalence of the A2143G, A2144G, and A2143C point mutations among H. pylori strains from gastric biopsy specimens in Bushehr, in the southwest of Iran.

Patients and Methods: Gastric biopsy specimens were obtained from patients with upper gastrointestinal symptoms during endoscopy. Fluorescent in situ hybridization (FISH) using oligonucleotide probes was applied to detect the point mutations responsible for clarithromycin resistance in H. pylori.

Results: Of the 135 H. pylori-positive specimens, two harbored strains with the A2143G mutation and nine contained strains with the A2144G mutation. Thus, the prevalences of the A2143G and A2144G point mutations were 1.5% and 6.7%, respectively. The A2143C point mutation was not found.

Conclusions: The prevalences of the point mutations A2143G and A2144G were low in our geographic area. Based on the findings of this study, clarithromycin still seems to be a useful antibiotic for initial treatment regimens in Bushehr, Iran.

Keywords: Clarithromycin Resistance, Point Mutation, Helicobacter pylori
Copyright © 2016, Hamadan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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