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Submitted: 09 Aug 2015
Revision: 22 Aug 2015
Accepted: 19 Sep 2015
ePublished: 23 Nov 2015
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Avicenna J Clin Microbiol Infect. 2015;2(4): 31990.
doi: 10.17795/ajcmi-31990
  Abstract View: 1356
  PDF Download: 1258

Research Article

Purification, Extraction and Visualization of Lipopolysaccharide of Escherichia coli From Urine Samples of Patients With Urinary Tract Infection

Fariha Akhter Chowdhury 1, Mohammad Nurul Islam 1, Anamika Saha 1, Sabrina Mahboob 1, Abu Syed Md. Mosaddek 2, Md Omar Faruque 3, Most. Fahmida Begum 4, Rajib Bhattacharjee 1*

1 Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
2 Department of Pharmacology, Uttara Adhunik Medical College, Dhaka, Bangladesh
3 Department of Physiology and Molecular Biology, Bangladesh University of Health Sciences, Dhaka, Bangladesh
4 Department of Microbiology, Uttara Adhunik Medical College, Dhaka, Bangladesh
*Corresponding Author: Corresponding author: Rajib Bhattacharjee, Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh. Tel: +1-7807079527, Fax: +880-255668202, Email: rajibbhattacharjee@yahoo.com

Abstract

Background: Urinary tract infection (UTI) is one of the most common infectious diseases in Bangladesh where Escherichia coli is the prevalent organism and responsible for most of the infections. Lipopolysaccharide (LPS) is known to act as a major virulence factor of E. coli.

Objectives: The present study aimed to purify, extract and visualize LPS of E. coli clinical isolates from urine samples of patients with UTI.

Patients and Methods: The E. coli strain was isolated from the urine samples of 10 patients with UTI and then the antibiotic sensitivity pattern of the isolates was determined. The purification of LPS was carried out using the hot aqueous-phenol method and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, which was directly stained using the modified silver staining method and Coomassie blue.

Results: The silver-stained gel demonstrated both smooth and rough type LPS by showing trail-like band patterns with the presence and lacking O-antigen region, respectively. Coomassie blue staining showed no band assuring the absence of any contaminating protein.

Conclusions: Our successful extraction of purified LPS from E. coli isolates of UTI patients’ urine samples can be an important step to understand the UTI disease conditions.


Copyright © 2015, Hamadan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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