Submitted: 29 Sep 2014
Revised: 04 Dec 2014
Accepted: 17 Dec 2014
First published online: 20 Feb 2015
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Avicenna J Clin Microbiol Infect. 2015;2(1): e23233.
doi: 10.17795/ajcmi-23233
  Abstract View: 535
  PDF Download: 327

Research Article

Isolation and Identification of Bacillus Species From Soil and Evaluation of Their Antibacterial Properties

Mansour Amin 1, Zeinab Rakhisi 2 * , Amanollah Zarei Ahmady 3

1 Health Research Institute, Infectious and Tropical Diseases Research Center, Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
2 School of Basic Sciences, Islamic Azad University, Fars Science and Research Branch, Shiraz, IR Iran
3 Nanotechnology Research Center, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
Corresponding author: Zeinab Rakhisi, School of Basic Sciences, Islamic Azad University, Fars Science and Research Branch, Shiraz, IR Iran. Tel: +98-9166319380 Email: zeinabrakhisi@yahoo.com


Background: Bacillus species are the predominant soil bacteria because of their resistant-endospore formation and production of essential antibiotics such as bacitracin.

Objectives: The aim of this study was to isolate Bacillus spp. from riverside soil and investigate their antimicrobial characteristics against some pathogenic bacteria.

Materials and Methods: Fifty soil samples were collected from different sites of Bahmanshir riverside in Abadan city, Iran, and analyzed for the presence of Bacillus species. The media used in this research were nutrient broth and agar. Bacillus species were identified by their phenotypic and biochemical characteristics. The antimicrobial effects of Bacillus extract against the target bacteria including Escherichia coli, Staphylococcus aureus, Salmonella typhi, Shigella dysenteriae and Corynebacterium diphtheriae were examined.

Results: The identified Bacillus species included B. cereus (86.6%), B.subtilis (6.6%), B. thuringiensis (3.3%), and B. pumilus (3.3%). Evaluation of the antimicrobial activity of the extracted compounds was carried out against five different bacteria. Antibiotic production tests indicated that two Bacillus strains belong to B. cereus, which showed antimicrobial properties. The minimum inhibitory concentrations (MICs) of these compounds ranged between 8.34-33.34 mg/mL for the target bacteria.

Conclusions: This study indicated that some Bacillus species have the potential to produce antimicrobial compounds which can be used to control microbial infections.

Keywords: Soil, Bacteria, Anti-Infective Agents, Bacillus
Copyright © 2015, Hamadan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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