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Submitted: 09 Aug 2021
Revision: 26 Nov 2021
Accepted: 25 Dec 2021
ePublished: 29 Mar 2022
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Avicenna J Clin Microbiol Infect. 2022;9: 16-20.
doi: 10.34172/ajcmi.2022.03
  Abstract View: 27
  PDF Download: 21

Original Article

The Antibacterial Analysis of Alcohol-Free and Alcohol-Based Chlorhexidine Mouthwashes Against Oral Bacteria

Hojatollah Yousefimanesh 1* ORCID logo, Maryam Robati 2, Atefeh Piri 3, Azardokht Khosravi Boroujeni 4, Mehrandokht Sirous 5

1 Department of Periodontology, School of Dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2 Department of Oral Medicine, School of Dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3 Department of Periodontology, School of Dentistry, Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4 Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
5 Department of Microbiology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
*Corresponding Author: *Corresponding author: Hojatollah Yousefimanesh, Assistant Professor, Department of Periodontology, School of Dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Tel: +989304874007, Email: , Email: hojjatyoosefi@yahoo.com

Abstract

Background: Chlorhexidine (CHX) is the gold standard chemical agent against oral pathogenic bacteria and is widely used for plaque/gingivitis control. The aim of the present study was to compare the effect of alcohol-based and alcohol-free CHX mouthwashes on oral microorganisms.

Methods: In the present in vitro study, the standard strains of four microorganisms present in the oral cavity were prepared, including Streptococcus mutans, Streptococcus sanguinis, Streptococcus salivarius, and Lactobacillus casei. The serial dilutions of CHX antimicrobial agents were obtained, and the level of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was determined using the broth dilution method. Finally, data were analyzed using the Kruskal-Wallis test, the Mann-Whitney U test, and SPSS-16.

Results: The MIC values of 0.12% and 0.2% alcohol-free CHX and 0.2% alcohol-based CHX for S. mutans were 1.17, 0.48, and 0.24 µg/mL, respectively. The MBC values of 0.12% and 0.2% alcohol-free CHX and 0.2% alcohol-based CHX for S. mutans were 18.78, 7.81, and 7.81 µg/mL, respectively. The MIC and MBC values of the tested CHX mouthwashes for S. mutans were significant (P≤0.05).

Conclusions: Overall, the 0.2% alcohol-based CHX mouthwash had the highest antibacterial activity against gram-positive bacteria.

Keywords: Chlorhexidine, Mouthwashes, Minimum inhibitory concentration, Minimum bactericidal concentration, Alcohol

Please cite this article as follows: Yousefimanesh H, Robati M, Piri A, Khosravi Boroujeni A, Sirous M. The antibacterial analysis of alcoholfreeand alcohol-based chlorhexidine mouthwashes against oral bacteria. Avicenna J Clin Microbiol Infect. 2022; 9(1):16-20. doi:10.34172/ajcmi.2022.03
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