Submitted: 06 Nov 2014
Revision: 28 Nov 2014
Accepted: 17 Dec 2014
ePublished: 14 Feb 2015
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Avicenna J Clin Microbiol Infect. 2015;2(1): 25171.
doi: 10.17795/ajcmi-25171
  Abstract View: 513
  PDF Download: 292

Research Article

The Comparison of Staphylococcus aureus Isolated From Blood and Wound Specimens for Genes Encoding Polysaccharide Intercellular Adhesion (PIA)

Abdolmajid Ghasemian 1, Shahin Najar-Peerayeh 1*, Bita Bakhshi 1

1 Department of Bacteriology, Tarbiat Modares University, Tehran, IR Iran
Corresponding author: Shahin Najar-Peerayeh, Department of Bacteriology, Tarbiat Modares University, Tehran, IR Iran. Tel: +98-92182883870, Fax: +98-2182884555 najarp_s@ modares.ac.ir


Background: The polysaccharide intercellular adhesion (PIA) produced by Staphylococcus aureus is effective in the protection of isolates from outer harsh conditions and progress of infection.

Objectives: The aim of this study was to compare the icaABCD genes encoding polysaccharide intercellular adhesion (PIA) between blood and wound isolates of Staphylococcus aureus (S. aureus) in Tehran.

Patients and Methods: Forty-eight clinical isolates (including 30 blood and 18 skin wounds) were collected from patients and were identified. Next, mecA gene, SCCmec types and icaABCD genes were detected among blood and wound isolates of S. aureus by PCR assay and specific primers.

Results: Nine (19%) out of 12 methicillin resistant S. aureus (MRSA) isolates harbored SCCmec type III and three (6.2%) isolates harbored SCCmec type V. Prevalence of icaA, icaB, icaC and icaD in blood isolates was twenty-one (70%), fourteen (48%), nineteen (64%) and eighteen (60%), respectively; while the prevalence in wound isolates was as nine (50%), seven (39%), six (34%) and twelve (67%), respectively.

Conclusions: These findings showed no significant difference regarding the presence of icaADBC genes between blood and wound isolates.

Keywords: Methicillin-Resistant, Staphylococcus aureus, Biofilm
Copyright © 2015, Hamadan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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