Logo-ajcmi
Submitted: 06 Nov 2014
Revised: 28 Nov 2014
Accepted: 17 Dec 2014
First published online: 14 Feb 2015
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - FireFox Plugin)

Avicenna J Clin Microbiol Infect. 2015;2(1): e25171.
doi: 10.17795/ajcmi-25171
  Abstract View: 144
  PDF Download: 145

Research Article

The Comparison of Staphylococcus aureus Isolated From Blood and Wound Specimens for Genes Encoding Polysaccharide Intercellular Adhesion (PIA)

Abdolmajid Ghasemian 1, Shahin Najar-Peerayeh 1 * , Bita Bakhshi 1

1 Department of Bacteriology, Tarbiat Modares University, Tehran, IR Iran
Corresponding author: Shahin Najar-Peerayeh, Department of Bacteriology, Tarbiat Modares University, Tehran, IR Iran. Tel: +98-92182883870, Fax: +98-2182884555 Email: najarp_s@ modares.ac.ir

Abstract

Background: The polysaccharide intercellular adhesion (PIA) produced by Staphylococcus aureus is effective in the protection of isolates from outer harsh conditions and progress of infection.

Objectives: The aim of this study was to compare the icaABCD genes encoding polysaccharide intercellular adhesion (PIA) between blood and wound isolates of Staphylococcus aureus (S. aureus) in Tehran.

Patients and Methods: Forty-eight clinical isolates (including 30 blood and 18 skin wounds) were collected from patients and were identified. Next, mecA gene, SCCmec types and icaABCD genes were detected among blood and wound isolates of S. aureus by PCR assay and specific primers.

Results: Nine (19%) out of 12 methicillin resistant S. aureus (MRSA) isolates harbored SCCmec type III and three (6.2%) isolates harbored SCCmec type V. Prevalence of icaA, icaB, icaC and icaD in blood isolates was twenty-one (70%), fourteen (48%), nineteen (64%) and eighteen (60%), respectively; while the prevalence in wound isolates was as nine (50%), seven (39%), six (34%) and twelve (67%), respectively.

Conclusions: These findings showed no significant difference regarding the presence of icaADBC genes between blood and wound isolates.

Keywords: Methicillin-Resistant, Staphylococcus aureus, Biofilm
Copyright © 2015, Hamadan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
First name
 
Last name
 
Email address
 
Comments
 
Security code


Article Viewed: 144

Your browser does not support the canvas element.


PDF Downloaded: 145

Your browser does not support the canvas element.