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Submitted: 16 Aug 2014
Revised: 23 Aug 2014
Accepted: 28 Aug 2014
First published online: 19 Oct 2014
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Avicenna J Clin Microbiol Infect. 2014;1(3): e22802.
doi: 10.17795/ajcmi-22802
  Abstract View: 228
  PDF Download: 274

Research Article

Prevalence and Resistance Profiles of Enteropathogenic and Shiga ToxinProducing Escherichia coli in Diarrheic Calves in Mashhad and Garmsar Districts, Iran

Mahdi Askari Badouei 1 * , Samad Lotfollahzadeh 2, Moein Arman 3, Masoud Haddadi 3

1 Department of Pathobiology, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, IR Iran
2 Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, IR Iran
3 Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, IR Iran
Corresponding author: Mahdi Askari Badouei, Department of Pathobiology, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, IR Iran. Tel: +98- 2334252121, Fax: +98-2334252020 Email: askari@iau-garmsar.ac.ir, mic.consult@gmail.com

Abstract

Background: Shiga toxin-producing Escherichia coli (STEC) strains are considered as one of the most important widespread food-borne pathogens, which cause diarrhea and life threatening diseases, such as hemolytic uremic syndrome, in humans. More recently, the STEC strains have also been incriminated to cause diarrhea and hemorrhagic colitis in calves; enteropathogenic E. coli (EPEC) also causes diarrhea in neonate animals.

Objectives: This study aimed to study the prevalence and antibacterial resistance patterns of STEC and EPEC in fecal samples from diarrheic calves in Mashhad and Garmsar districts, Iran.

Materials and Methods: A total of 115 fecal samples were collected from diarrheic animals, 75 from Mashhad and 40 from Garmsar districts. A total of 146 E. coli isolates were obtained from culture and subjected to multiplex-PCR assay targeting stx1, stx2, eaeA and ehly virulence genes. The antibacterial resistance patterns of the virulence-positive isolates were determined using disc diffusion method.

Results: Eight samples (6.9%) carried the strains with positive results for at least one of the tested virulence genes. Five samples (4.3%) contained the stx-positive strains (STEC) and three (2.6%) carried the eaeA-positive and stx-negative strains, which were categorized as EPEC. In nine virulence-positive E. coli isolates, stx1 (n = 6) was the predominant virulence gene, followed by ehly (n = 5), eae (n = 4), and stx2 (n = 2). Antibacterial resistance patterns of virulence-positive isolates were also determined and nine resistance profiles were discriminated; higher rates of resistance were observed in isolates from Mashhad.

Conclusions: This study indicated that other pathologic factors might play a more important role in calf diarrhea in the studied areas, but public health significance of these strains should not be overlooked.

Keywords: STEC, EPEC, Calf Diarrhea, Antibacterial Resistance
Copyright © 2014, Hamadan University of Medical Sciences; Published by Safnek. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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