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Submitted: 08 Dec 2018
Accepted: 19 Feb 2019
First published online: 28 Feb 2019
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Avicenna J Clin Microbiol Infect. 2019;6(1):15-20.
doi: 10.15171/ajcmi.2019.04
  Abstract View: 60
  PDF Download: 67

Original Article

The New Insight for Novel Antimicrobial Peptides Designing by Computational Design and Improvement of an Antimicrobial Peptide Derivate of LL-37

Masoud Keikha 1,2 ORCID logo, Hossein-Ali Rahdar 3, Morteza Karami-Zarandi 3, Davood Azadi 4, kiarash Ghazvini 1 * ORCID logo

1 Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2 Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
3 Department of Medical Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
4 Department of Laboratory Sciences, Khomein University of Medical Sciences, Khomein, Iran

Abstract

Background: LL-37 is one of the well-known antimicrobial peptides which is effective on the extended expecetrome of microbial pathogens. The aim of this study was to design a modified digital analog of LL-37 with enhanced antimicrobial activity and restricted toxicity of the host cell.

Methods: Online databases and software were used for determining LL-37 characteristics such as hydrophobicity, Booman index, and hemolysis probability. Variant structures based on the replacement of leucine and lysine with tryptophan and arginine were calculated as well. Finally, the best sequence was selected and analyzed for approving as an antimicrobial peptide.

Results: The antibacterial characteristics of LL-37 were improved by replacing the arginine and tryptophan and according to systemic calculations, it was defined that this peptide is an antimicrobial peptide by 97% confidential.

Conclusions: In general, bioinformatics tools are considered as one of the most available and efficient tools for antimicrobial peptide designing. Therefore, future studies could use kLL-39 as an antimicrobial peptide and investigate its antimicrobial effects in vitro.

Keywords: LL-37, Antimicrobial peptides, Computational studies, Infectious disease
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