Submitted: 08 May 2019
Accepted: 09 Jul 2018
First published online: 27 Aug 2018
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Avicenna J Clin Microbiol Infect. 2018;5(3):41-45.
doi: 10.15171/ajcmi.2018.08
  Abstract View: 76
  PDF Download: 75

Research Article

Coagulase Gene Polymorphism of Staphylococcus Aureus Isolates Collected From Human Clinical Samples in Southeastern Iran

Mousa Sheikh 1 ORCiD, Mohammad Moradi 2, zahraZ Rashki Ghalehnoo 3 * ORCiD, saeed salari 4

1 Department of Basic Sciences, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
2 Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
3 Department of Microbiology, Faculty of Medicine, Zabol University of Medical Sciences, Zabol, Iran
4 Department of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
*Corresponding author: Zahra Rashki Ghalehnoo, Department of Microbiology, Faculty of Medicine, Zabol University of Medical Sciences, Zabol, Iran. Tel.: +98 915 1971410; Fax: +98 5432232191. Email: Email: zahrarashki@yahoo.co.uk

Article

Background: Staphylococcus aureus is one of the most important pathogens in the development of hospital infections. The production of coagulase is directly related to the pathogenicity of S. aureus. The present study was conducted in order to investigate the polymorphism of the coagulase gene (coa) as a coagulase-encoding gene.

Methods: 120 clinical samples were collected from patients admitted to Imam Khomeini and Amiralmomenin hospitals in Zabol, southeastern Iran, during 2014 and screened for the presence of S. aureus. Genetic diversity was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) of coa gene and digested by AluI enzyme.

Results: Out of 120 clinical samples, 56 isolates were diagnosed as S. aureus and all of these isolates were positive for the 3′ end of the coa gene by PCR. All isolates produced a single band on coa PCR, with molecular sizes ranging from 600 to 850 bp, with the highest frequency of 700 bp (39%). RFLP analysis of coa gene using AluI revealed P1-P4 band patterns with the highest frequency of P1.

Conclusions: The results of this study showed that P1 pattern was the most frequent pattern and lowest frequency was observed in P4 pattern. The results of this study suggest that the RFLP of coa gene can be used as a fairly suitable method for determining the genetic differences between S. aureus isolates. Keywords: Staphylococcus aureus, coa gene, RFLP

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