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Submitted: 01 Mar 2014
Revised: 20 Mar 2014
Accepted: 01 Jun 2014
First published online: 01 Aug 2014
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Avicenna J Clin Microbiol Infect. 2014;1(2): e21034.
doi: 10.17795/ajcmi-21034
  Abstract View: 162
  PDF Download: 145

Research Article

Comparison of Real-Time Polymerase Chain Reaction and Conventional Cell Culture for Detection of Influenza A in Tabriz, Iran

Sirus Jedary Seif 1, Masoud Sabouri Ghannad 2 *

1 Department of Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IR Iran
2 Research Center for Molecular Medicine, Department of Microbiology, Research Center for Molecular Medicine, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran
Corresponding author: Masoud Sabouri Ghannad, Research Center for Molecular Medicine, Department of Microbiology, Research Center for Molecular Medicine, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, P.O.BOX: 6517838736, IR Iran. Tel: +98-8138380160, Fax: +98-8138380208 Email: sabouri@umsha.ac.ir

Abstract

Background: Influenza type A (H1N1) causes an epidemic disease, resulting in thousands of deaths throughout the world.

Objectives: Our aim was comparing the efficacy of two different methods in isolating the Influenza (H1N1) virus including cell culture and real-Time PCR in Tabriz and suburbs.

Patients and Methods: Of throat swab samples, 220 were collected in viral transport medium (VTM) from patients with a suspected influenza virus infection referred to hospitals and clinics of Tabriz University of Medical Sciences. All the samples were examined through virus culturing in Madin-Darby canine kidney epithelial cells (MDCK line) and also real-time polymerase chain reaction (RT-PCR). The collected data were analyzed by SPSS version 16.

Results: RT-PCR detected 41 cases of influenza A (H1N1), compared with the virus isolation methods that detected 18 cases.

Conclusions: RT-PCR provided a sensitive and specific route compared with virus isolation. In addition, urgent planning for the vaccination program of influenza A is suggested, which can definitely prevent the spread of virus in this part of Iran.

Keywords: Influenza a Virus, H1N1 Subtype, Real-Time Polymerase Chain Reaction, epidemics, Pandemics
Copyright © 2014, Hamadan University of Medical Sciences; Published by Safnek. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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