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Submitted: 05 May 2014
Revised: 29 Jun 2014
Accepted: 29 Jun 2014
First published online: 01 Aug 2014
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Avicenna J Clin Microbiol Infect. 2014;1(2): e20208.
doi: 10.17795/ajcmi-20208
  Abstract View: 223
  PDF Download: 234

Research Article

Enterotoxin and Exfoliative Toxin Genes Among Methicillin-Resistant Staphylococcus aureus Isolates Recovered From Ilam, Iran

Parisa Asadollahi 1, Ali Delpisheh 2, Mohammad Hossein Maleki 3, Farid Azizi Jalilian 3,4, Mahammad Yosuf Alikhani 5, Khairollah Asadollahi 2, Setareh Soroush 3,4, Ali Hematian 4, Mohammad Emaneini 1, Morovat Taherikalani 3,4 *

1 Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran
2 Department of Epidemiology, School of Medicine, Ilam University of Medical Sciences, Ilam, IR Iran
3 Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, IR Iran
4 Department of Microbiology, School of Medicine, Ilam University of Medical Sciences, Ilam, IR Iran
5 Brucelosis Reseach Center and Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran
Corresponding author: Morovat Taherikalani, Department of Microbiology, School of Medicine and Clinical Microbiology Research Center, Ilam University of Medical Sciences, Banganjab, IR Iran. Tel: +98-8412235747; Fax: +98-8412227134 Email: alikhani43@yahoo.com

Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is one the most important pathogens across the world, associated with increased mortality rate compared to other Staphylococcal species.

Objectives: The present study aimed to investigate different virulence determinants among MRSA isolates from Ilam, Iran.

Materials and Methods: Overall, 100 MRSA isolates were collected from selected hospitals of Ilam, Iran. Oxacillin screening test and molecular detection of mecA were carried out by polymerase chain reaction (PCR) to confirm the methicillin resistance. Presence of the virulence genes et A, B, tst, sea a, b, c, d and e were assessed using multiplex PCR.

Results: All the tested isolates were susceptible to vancomycin, but resistant to penicillin (100%), erythromycin (31%), clindamycin (27%), ciprofloxacin (27%), gentamicin (21%), and amikacin (16%). The frequencies of antibiotic resistance and virulence genes were as follows: tst 46%, eta 1%, etb 3%, sea 41%, seb 2%, sec 14%, and see 31%.

Conclusions: Results of the present study showed that the tst and sea genes had high frequencies among the MRSA isolates. The increased prevalence of MRSA isolates containing different virulence genes, probably accompanied by antimicrobial resistance, can complicate the therapy of MRSA isolates.

Keywords: Methicillin-Resistant, Staphylococcus aureus, Virulence Determinant
Copyright © 2014, Hamadan University of Medical Sciences; Published by Safnek. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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