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Submitted: 01 May 2014
Revised: 30 Jun 2014
Accepted: 01 Jul 2014
First published online: 30 Aug 2014
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Avicenna J Clin Microbiol Infect. 2014;1(2): e19908.
doi: 10.17795/ajcmi-19908
  Abstract View: 220
  PDF Download: 207

Research Article

Inhibitory Effect of Lactobacillus reuteri on Some Pathogenic Bacteria Isolated From Women With Bacterial Vaginosis

Gita Eslami 1, Raheleh Karimiravesh 1 * , Sudabeh Taheri 1, Eznollah Azargashb 2

1 Department of Microbiology, Faculty of Medicine, Shahid Beheshti University, Tehran, IR Iran
2 Department of Community Medicine, Faculty of Medicine, Shahid Beheshti University, Tehran, IR Iran
Corresponding author: Raheleh Karimiravesh, Department of Microbiology, Faculty of Medicine, Shahid Beheshti University, Tehran, IR Iran. Tel: +98-939-2206484 Email: ra.karimiravesh@gmail.com

Abstract

Background: Considering the high prevalence of bacterial vaginosis and its association with urinary tract infection in women and treatment of gynecologic problems occur when a high recurrence of bacterial vaginosis is often treated with antibiotics.

Objectives: The purpose of this study was to investigate the inhibitory effect of Lactobacillus reuteri on pathogenic bacteria isolated from women with bacterial vaginosis.

Materials and Methods: Ninety-six samples were obtained from vaginal discharge of women with bacterial vaginosis by a gynecologist with a Dacron swab and put in sterile tubes containing TSB broth and Thioglycollate broth. Then were immediately sent to the laboratory in cold chain for further assessment. Afterward, culture was transferred on blood agar, EMB, Palcam and differential diagnosis environments. Then cultures were incubated for 24 hours at 37 °C. Lactobacillus reuteri strains were cultured in MRS environment and transferred to laboratory. After purification of pathogenic bacteria, Lactobacillus reuteri inhibitory effect on pathogenic bacteria was evaluated by minimum inhibitory concentration (MIC) and antibiogram. Statistical analysis was performed using SPSS software v.16.

Results: The results of this study demonstrated the inhibitory effect of Lactobacillus reuteri on some pathogenic bacteria that cause bacterial, including Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Streptococcus agalactiae, Enterococcus, Listeria monocytogenes and E. coli. Microscopic examination of stained smears of most Lactobacillus and pathogenic bacteria showed reduced. The prevalence of abnormal vaginal discharge, history of drug use, contraceptive methods and douching were 61%, 55%, 42% and 13%, respectively. Significant difference was observed between the use and non-use of IUD in women with bacterial .

Conclusions: Our findings indicated the inhibitory effect of Lactobacillus reuteri on pathogenic bacteria that cause bacterial vaginosis. The results of this study confirmed the inhibitory effect of probiotics on pathogens growth that cause bacterial vaginosis, which can be considered in the prevention and treatment of bacterial vaginosis in further investigations.

Keywords: Lactobacillus reuteri, probiotics, bacterial
Copyright © 2014, Hamadan University of Medical Sciences; Published by Safnek. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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