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Submitted: 12 Jul 2018
Accepted: 05 Aug 2018
First published online: 27 Aug 2018
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Avicenna J Clin Microbiol Infect. 2018;5(3):67-70.
doi: 10.15171/ajcmi.2018.13
  Abstract View: 164
  PDF Download: 135

Original Article

Characterization of Staphylococcus aureus Isolated From Wound Infectious in Diabetes Clinic of Hazrat Fatemeh Zahra (SA) Hospital

Vajiheh Nourbakhsh 1 ORCID logo, Fahimeh Nourbakhsh 2 * ORCID logo, Elaheh Tajbakhsh 3, Samaneh Borooni 4, Dana Daneshmand 5

1 Hazrat Fatemeh Zahra (SA) Hospital, Depending on the Treatment and Management of Social Security in Isfahan, Isfahan, Iran
2 Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
3 Department of Microbiology, Faculty of Basic Science, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
4 Nourdanesh Institute of Higher Education of Meymeh, Isfahan, Iran
5 Nosocomial Infection Research Center, Isfahan University of Medical Science, Isfahan, Iran
*Corresponding author: Fahimeh Nourbakhsh, Ph.D. Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, P. O. Box, 1365-91775, I.R. Iran. Phone: +98-51-3180-1176 Email: Email: Nourbakhsh951@mums.ac.ir

Abstract

Background: Staphylococcus aureus is a gram positive pathogen which causes a wide range of infections. The present study aimed to investigate genotypic and phenotypic screening about biofilm formation in S. aureus isolated from wound infections in a diabetes clinic in Hazrat Fatemeh Zahra (SA) hospital.

Methods: A total of 267 clinical samples were collected from various types of wound infections in the diabetes clinic of Hazrat Fatemeh Zahra (SA) hospital, Isfahan, Iran. The methicillin-resistant S. aureus (MRSA) isolates were selected and biofilm formation and its related genes were analyzed by polymerase chain reaction (PCR).

Results: The results showed that 95 out of 132 samples were MRSA. The high resistance was seen to methicillin, erythromycin, ciprofloxacin, and penicillin. Phenotypic results showed that 48.3% of the isolates were high biofilm producers, 29.1% were average biofilm producers, and 10.6% were low biofilm producers.

Conclusions: According to the results of this study, the expression levels of biofilm-associated genes significantly increased, and high prevalence of antibiotic resistance was one of the important reasons for the development of drug resistance in patients.

Keywords: Staphylococcus aureus, Methicillin resistance, Biofilm, Multiplex PCR, Iran
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