Submitted: 13 Mar 2018
Accepted: 08 Nov 2018
First published online: 29 Dec 2018
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Avicenna J Clin Microbiol Infect. 2018;5(4):91-96.
doi: 10.15171/ajcmi.2018.19
  Abstract View: 18
  PDF Download: 28

Research Article

SCCmec Typing and the Association of pvl, ACME, sea and seb Genes in Staphylococcus aureus Isolates From Burn Wound Infections

Hossein Motamedi 1,2 * , Elahe Soltani Fard 1, Mahshid Aria 1, Seyyed Mojtaba Moosavian 3

1 Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Biotechnology and Biological Science Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Department of Microbiology, Faculty of Medicine, JundiShapur University of Medical Sciences
Hossein Motamedi, Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Golestan Blvd., Daneshgah sq., Ahvaz, Khouzestan, Iran. Tel/fax: +98-6133331045 Email: motamedih@scu.ac.ir

Article

Background: Staphylococcus aureus is a very important hospital and community-acquired pathogen that is prevalent in burn wounds, so finding the sources of contamination and infection with it are important for its control. The aim of this study was to do SCCmec typing and determine the prevalence of pvl, ACME, sea and seb genes among S. aureus isolates from burn patients.

Methods: Fifty S. aureus isolates were sampled from burn patients and were identified. These isolates were typed based on SCCmec typing and detection of mecA and pvl genes using multiplex polymerase chain reaction (PCR) method and also were screened for the presence of ACME, sea and seb genes by PCR.

Results: Three isolates were identified as methicillin-resistant strains. The sea gene was identified in 22% of isolates while pvl, ACME and seb genes were not found in the isolates. Multiplex PCR SCCmec typing of 3 methicillin resistant S. aureus (MRSA) isolates showed that 2 isolates belong to SCCmec type II and 1 isolate to type I.

Conclusions: The results suggest that methicillin resistant S. aureus isolates are prevalent in burn patients and mecA gene is involved in resistance to methicillin. These strains belonged to SCCmec type II and I which can affect horizontal gene transfer among S. aureus isolates and hence distribution of virulence genes. Therefore, it is necessary to frequently monitor S. aureus isolates through typing and screening virulence genes to control this pathogen.

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