Submitted: 22 Sep 2014
Revised: 01 Dec 2014
Accepted: 17 Dec 2014
First published online: 14 Feb 2015
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Avicenna J Clin Microbiol Infect. 2015;2(1): e23621.
doi: 10.17795/ajcmi-23621
  Abstract View: 45
  PDF Download: 42

Research Article

Screening of Actinomycetes From Lipar Area of Oman Sea to Investigate the Antibacterial Compounds

Mahsa Shams 1, Bahar Shahnavaz 1 * , Kiarash Ghazvini 2, Toraj Valinasab 3

1 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, IR Iran
2 Department of Microbiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran
3 Iranian Fisheries Research Organization, Tehran, IR Iran
Corresponding author: Bahar Shahnavaz, Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, IR Iran. Tel/Fax: +98-5138762227 Email: shahnavaz@ um.ac.ir

Abstract

Background: Actinomycetes are one of the most important sources for the production of antibacterial compounds. Marine environments, due to their unique characteristics, are considered a good option to search for bacteria with the capability of producing antimicrobial compounds.

Objectives: The purpose of this study was to isolate the actinomycetes producing antibacterial compounds.

Materials and Methods: A total of 35 actinomycetes were isolated from Oman Sea (Lipar Area). To investigate antibacterial activity, the isolated actinomycetes were assessed against reference and pathogenic bacteria, including Staphylococcus epidermidis, Staphylococcu intermedius, Staphylococcu chromogenes, Staphylococcu saprophyticus, Bacillus cereus and methicillin-resistance Staphylococcu aureus, Pseudomonas, Listeria, Klebsiella, Salmonella, Acinetobacter, and Escherichia coli O157:H7, using the cross streak method.

Results: Based on the morphological characterization, 35 isolated cases belonged to actinomycetes and %94 of them had the ability to produce antibacterial compounds. In the cross streak method, most of the isolated bacteria have antibacterial activity against reference S. aureus among Gram-positive bacteria and Acinetobacter among Gram-negative bacteria. Inhibition zone diameters were measured between 2-25 and 1-20 mm for Gram-positive and -negative bacteria, receptivity.

Conclusions: Preliminary results indicate that the native Iranian Actinobacteria could be considered a suitable option for screening of the new antibacterial compounds. Molecular research and antibacterial compound extraction against the aforementioned pathogenic strains are also being conducted.

Copyright © 2015, Hamadan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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