Submitted: 22 Aug 2014
Revised: 09 Sep 2014
Accepted: 11 Sep 2014
First published online: 22 Oct 2014
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Avicenna J Clin Microbiol Infect. 2014;1(3): e22981.
doi: 10.17795/ajcmi-22981
  Abstract View: 78
  PDF Download: 72

Research Article

Comparison of the Common Adhesin Coding Operons Distribution in Uropathogenic and Phylogenetic Group B2 and A Escherichia coli Isolates 

Masoud Rahdar 1, Ahmad Rashki 2 * , Hamidreza Miri 1

1 Department of Biology, Faculty of Basic Science, University of Zabol, Zabol, IR Iran
2 Department of Physiopathology, Faculty of Vet-Medicine, University of Zabol, Zabol, IR Iran
Corresponding author: Ahmad Rashki, Department of Physiopathology, Faculty of Vet-Medicine, University of Zabol, Zabol, IR Iran. Tel: +98-9151970877, Fax: +98-5424822251 Email: ah_rashki@usal.es

Article

Background: Escherichia coli is one of the most causative pathogen of urinary tract infection. Urinary tract infections (UTIs) are the second most common cause of morbidity and remain a serious health concern among the clinicians. The severity of UTI caused by uropathogenic E. coli (UPEC) is due to the expression of a wide spectrum of virulent factors such as adhesin coding operons. Little is known about the relationship between the E. coli genetic background and the acquisition of adhesin coding operons in UPEC isolates.

Objectives: The aim of this study was to determine the prevalence of adhesin coding operons in UPEC isolates belonged to phylogenetic group B2 and A collected from patients suffering from UTI.

Materials and Methods: A total 100 UPEC isolates were used for DNA extraction by the boiling lysis. The analysis of phylogenetic groups, along with detection of adhesin coding operons was performed by Multiplex-PCR method. Associations were assessed between afa, fim, foc, pap and sfa operons among to 55 B2 and 17 A groups E. coli isolates. Statistical analysis was performed using Fisher exact test.

Results: Phylogenetic analysis showed that 55 and 17 of 100 UPEC isolates belonged to the B2 and A phylogenetic groups, respectively. The afa, fim, foc, pap and sfa operons were present in five (9.09%), 55 (100%), 16 (29.09%), 46 (83.63%) and 46 (83.63%) of UPEC isolates belonged to phylogenetic group B2, and two (12.50%), 14 (87.50%), one (6.25%), two (12.5%) and 12 (75%) of isolates belonged to phylogenetic group A, respectively. Statistical analysis showed that pap gene was significantly more frequently detected in phylogenetic group B2 (P < 0.05).

Conclusions: The UPEC isolates belonging to group B2 harbored a greater number of adhesin coding operons than strains from phylogenetic groups A.

Copyright © 2014, Hamadan University of Medical Sciences; Published by Safnek. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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