Submitted: 15 Sep 2017
Revised: 08 Jan 2018
Accepted: 14 Jan 2018
First published online: 17 Feb 2018
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Avicenna J Clin Microbiol Infect. 2018;5(1): e61772.
doi: 10.5812/ajcmi.61772
  Abstract View: 98
  PDF Download: 72

Research Article

Antibiofilm Activity of Dracocephalum polychaetum Extract on Methicillin-Resistant Staphylococcus aureus

Mohammad Mehdi Yaghoobi 1 * ORCiD, Mouj Khaleghi 2, Hajar Rezanejad 1, Paria Parsia 2

1 Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, IR Iran
2 Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, IR Iran
Corresponding author: Mohammad Mehdi Yaghoobi, Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, End of Haft-bagh Highway, P.O. Box: 7631818356, Kerman, IR Iran. Tel: +98-3431623209, Fax: +98-3433776617, Email: m.yaghoobi@kgut.ac.ir

Article

Background: Antibiotic resistance among biofilm-producing pathogenic bacteria is a major health concern today. Plants, as a rich source of medicinal compounds, are interestingly explored for discovering new antibiotics.

Objectives: In this study, the effect of Dracocephalum polychaetum Bornm extract on the growth, biofilm formation, and expression of biofilm-related genes in methicillin-resistant Staphylococcus aureus (MRSA) was explored.

Methods: Antimicrobial activity of D. polychaetum aerial part extract in MRSA samples was evaluated by agar well diffusion method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract were determined according to the CLSI manual. Inhibition of biofilm formation was analyzed by microtitre plate method. Expression of icaA, icaD, bap, sar, and agr genes was studied by Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) technique.

Results: The antimicrobial effect of the extract against 20 MRSA strains was appropriate. The MIC and MBC of the extract were in the range of 0.781 - 25 mg mL-1 and 1.56 - 50 mg mL-1, respectively. Biofilm formation was inhibited in all of the isolated MRSA strains in sub-MIC concentrations. PCR results demonstrated that half of the samples had both icaA and icaD genes, about 1/3 had icaA gene, and 1/6 had merely icaD gene. RT-qPCR data showed that the expression of sar, bap, icaD and icaA genes was significantly reduced by the extract.

Conclusions: Collectively, the results demonstrated that D. polychaetum not only had anti-staphylococcus effects against MRSA but also suppressed biofilm formation both at phenotype and gene expression levels. Its effects are comparable to the effect of other Dracocephalum sp. Further in vivo studies can reveal the potential application of this plant against MRSA strains.

Copyright © 2018, Avicenna Journal of Clinical Microbiology and Infection. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
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