Submitted: 10 May 2017
Revised: 06 Oct 2017
Accepted: 22 Nov 2017
First published online: 27 Nov 2017
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - FireFox Plugin)

AvicennaJ Clin Microbiol Infect. 2018;5(1): e12987.
doi: 10.5812/ajcmi.12987
  Abstract View: 14
  PDF Download: 15

Research Article

Antibacterial Activity of Azadirachta indica Leaf Extracts Against Some Pathogenic Standards and Clinical Bacterial Isolates

Leila Maleki 1, Tahereh Sadeghian-Rizi 2 * , Mostafa Ghannadian 3, Mohammad Hossein Sanati 4, Shahin Shafizadegan 5, Hojjat Sadeghi-Aliabadi 6 *

1 M.Sc. of Biotechnology, Department of Microbial Biotechnology, School of Biology Sciences, Nour Danesh Institute of Higher Education, Meymeh, Isfahan, Iran
2 Ph.D. Candidate in Pharmaceutical Biotechnology, Department of Pharmaceutical Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
3 Ph.D., Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
4 Ph.D., Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
5 B.SC of Biology, Department of Pharmaceutical Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
6 Ph.D., Department of Pharmaceutical Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
Corresponding author: Hojjat Sadeghi-Aliabadi, Department of Pharmaceutical Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran. Tel: +98-3137922564 Email: sadeghi@pharm.mui.ac.ir

Abstract

Background: Azadirachta indica A. Juss (Neem) has been used against a few diseases in traditional medicine. This study was conducted to evaluate the antibacterial activity of Neem leaves extract against some antibiotic sensitive and resistant strains of important human pathogenic bacteria.

Methods: The antimicrobial activities of ethanol, methanol, and ethyl acetate extracts of Azadirachta indica A. juss leaves were evaluated against some pathogenic bacterial strains including S. aureus ATCC 6538, E. faecalis ATCC 1394, P. aeroginosa ATCC 9027, E.coli, ATCC 25922, and their clinical isolates using agar well diffusion and MTT microdilution methods. MBC was determined by subculturing from microdilution assays.

Results: The results revealed that methanol extract has the strongest growth inhibitory effect on both standard and clinical isolated strains of P. aeroginosa. Ethyl acetate and ethanol extracts, showing a growth inhibitory effect on both standard and hospital isolated strains of S. aureus. In the case of E. faecalis, ethanol and methanol extracts showed the highest growth inhibitory effect against standard and clinical strains, respectively. According to the MIC index results, the methanol extract has a bactericidal activity against both standard and nosocomial strains of S. aureus and P. aeroginosa and bacteriostatic activity against nosocomial strain of E. faecalis. Ethanol extract showed bactericidal activity against both standard and nosocomial strains of E. faecalis and P. aeroginosa and bacteriostatic activity against nosocomial strain of S. aureus. Ethyl acetate extract had shown bactericidal activity against standard strains of S. aureus and P. aeroginosa and bacteriostatic against nosocomial strain of S. aureus and standard strain of E. faecalis.

Conclusions: Results revealed that Neem may be a prospective therapeutic agent to combat antibiotic resistant bacteria.

Copyright © 2017, Avicenna Journal of Clinical Microbiology and Infection. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
First name
 
Last name
 
Email address
 
Comments
 
Security code