Submitted: 28 Jun 2017
Accepted: 19 Mar 2018
First published online: 17 Apr 2018
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Avicenna J Clin Microbiol Infect. 2018;5(2):1-6.
doi: 10.15171/ajcmi.2018.01
  Abstract View: 14
  PDF Download: 23

Research Article

Evaluation of the Antimicrobial Effects of Nano-Essences of Allium sativum L., Thymus vulgaris L., Mentha piperita L., and Chamaemelum nobile L. on Common Causes of Otitis Externa

Samira Hassanzadeh Touri 1, Mansour Bayat 2 * ORCiD, Afshin Mohsenifar 3, Setareh Haghighat 1

1 Department of Microbiology, Pharmaceutical Sciences branch, Islamic Azad University, Tehran, Iran
2 Department of Microbiology, Faculty of Veterinary Specialized Sciences, Science and Research branch, Islamic Azad University, Tehran, Iran
3 Research and Development Center of Nanozino, Tehran, Iran
*Corresponding author: Mansour Bayat, Tel: +98 2144817170 Fax: +98 2144817175 Email: dr.mnbayat@gmail.com, Email: dr_ mansour_bayat@yahoo. com

Abstract

Background: Inflammation or infection of the ear that refers to as otitis is a common ear disorder. The otitis externa infection involves outer ear and ear canal. Streptococcus pyogenes, Staphylococcus aureus, and Pseudomonas aeruginosa can be pointed out as the common causative agents of this infection. Due to the detrimental effects of chemical drugs on humans, utilizing the new formulation of herbal essential oils, such as nano-encapsulation is considered owing to the more efficient and reduction of adverse effects resulting from the direct application of essential oils. In this in vitro study, the antimicrobial effects of 4 nano-essences were compared to pure essential oils against four major microorganisms involved in otitis externa (namely, S. pyogenes, S. aureus and P. aeruginosa).

Methods: To evaluate the antibacterial effects of nano-essences, minimum inhibitory concentration (MIC) quantitative determination and minimum bactericidal concentration (MBC) were first carried out, and then qualitative disk diffusion tests were conducted.

Results: The MIC quantitative test results demonstrated that among the evaluated essential oils, garlic nanoessence had the most antimicrobial effect at the lowest concentration. Besides, the results showed that nanoessences had lower effect compared to pure essential oils. Diffusion disc test results revealed that nano-essences could not be released from paper discs diffusion in solid media.

Conclusions: Results suggested great antibacterial effects of nano-essences of garlic, thyme, peppermint, and chamomile on 3 strains of bacteria involved in otitis externa and it can be promised to produce new drugs, with lower side effects in eliminating these pathogens.

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